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The effect of abiotic factors on house dust mites Dermatophagoides farinae
Vacková, Tereza ; Hubert, Jan (advisor) ; Zemek, Rostislav (referee)
House dust mites are one of the most important allergenic agents found in households worldwide. The biology of these mites is significantly affected by hygrothermal factors - especially by temperature and humidity. The study is focused on the influence of temperature and humidity to the behavior of Dermatophagoides farinae within a thermal gradient (simulating a vertical section of a mattress), on its metabolism (represented by respiration rate) and population growth. Within a constant thermal gradient (19-41 řC) run for 24 h D. farinae mites most often received food in the sector with a temperature range of 32-36 řC. In experiment with 5 days of alternating cycles of the same gradient (8 h / day) and room temperature (16 h / day) was the sector with a temperature range of 32-36 řC for mite's feeding also the most prefered. In contrast, in both variants of the experiment, non-feeding mites preferred the sector with a temperature range of 19-23 řC (or rather, the largest number of them were found in this sector during the evaluation). During the measurement of respiration, mites of this species showed significant CO2 production when were incubated at temperatures of 25- 30 řC. Out of the studied combinations of temperature and humidity, the highest respiration was measured when mites were incubated...
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