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Stabilization of bank of river
Odehnalová, Zuzana ; Ing.Pavla Pilařová (referee) ; Šlezingr, Miloslav (advisor)
The aim of this work is to design stabilizing the banks of the watercourse Svratka in the cadastral Tišnov. The chosen site was divided into ten sections. Each segment was classified in terms of the status of riparian vegetation. Stabilizing elements are designed in three sections chosen location, where banks are most stressed. In other parts do not necessarily represent a significant stabilization measures. The result is a proposal biotechnical stabilization, completed the design of riparian vegetation.
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Regulation of the river
Varga, Tomáš ; Pilařová, Pavla (referee) ; Šlezingr, Miloslav (advisor)
This bachelor thesis deals with the evaluation of current state of selected part of the river and deal with regulativ of river. The work is conceived on four different levels.The first on eis describesing general characteristics of the selected selection. Second concern calculation of capacity and following river regulativ. Next part contains proposal of vegetation belt. The last part is about drawing of current state and also river regulativ features.
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The development of an HPLC method for monitoring the preparation of oligodeoxynucleotide probes
Šemlej, Tomáš ; Kučera, Radim (advisor) ; Pilařová, Pavla (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Mgr. Tomáš Šemlej Supervisor: doc. PharmDr. Radim Kučera, Ph.D. Title of rigorosum thesis: The development of an HPLC method for monitoring the preparation of oligodeoxynucleotide probes An artificially prepared oligodeoxynucleotide sequence with the attached label is referred to as a molecular probe. Molecular probes are mainly used to quantify multiplication in RT-PCR and identify mutations in PCR analyses. There are typically two different types of probes - mono-labeled molecular probes and double-labeled molecular probes. The mono-labeled molecular probes are constructed from fluorophore- labeled oligodeoxynucleotide sequence or quencher-labeled oligodeoxynucleotide sequence. Whereas double-labeled molecular probes consist of an oligodeoxynucleotide sequence with a fluorophore attached at one end together with a quencher attached at the other end of the chain. In this work, we first paid attention to the separation of double- labeled oligodeoxynucleotides from mono-labeled oligodeoxynucleotides. Several types of stationary phases were tested and based on that the column Clarity® Oligo-RP™ was chosen to separate a mixture of labeled oligodeoxynucleotides. This...
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Use of liquid chromatography in pharmaceutical analysis II.
Sovič, Samuel ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Samuel Sovič Tutor: PharmDr. Pavla Pilařová, Ph.D. Title of Thesis: The use of liquid chromatography in pharmaceutical analysis II The diploma thesis deals with the analysis of the retention behavior of selected antiepileptics: Gabapentin, Levetiracetam, Primidone and Lamotrigine. Three different C18 stationary phases were tested such as Phenyl; PFP; Kinetex, different mobile phase composition, column temperature and suitable wavelengths in the range of 210-220 nm for all analytes. The optimal analysis conditions were on an Agilent Eclipse XDB-Phenyl 5 μm column (4.6 mm x 150 mm), at a temperature of 40 řC and a flow rate of MP at 0.8 ml/min, at a wavelength of 220 nm. By creating a gradient, the analysis was shortened from 15 minutes with isocratic elution to 9 minutes. The method was partially validated in terms of linearity, accuracy, precision, selectivity and robustness. After that, the method was applied for real samples of patients from FN HK.
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The influence of experimental conditions on chiral separation of borane clusters in capillary electrophoresis
Bodi, Adrián ; Kučera, Radim (advisor) ; Pilařová, Pavla (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of pharmaceutical chemistry and pharmaceutical analysis Candidate: Adrián Bodi Thesis supervisor: doc. PharmDr. Radim Kučera, Ph.D. Consultant: Mgr. Ondřej Horáček Title of thesis: The influence of experimental conditions on chiral separation of borane clusters in capillary electrophoresis Keywords: chiral separation, borane clusters, capillary electrophoresis, cyclodextrins Abstract: Boron compounds also include borane clusters, in which boron atoms form triangular configuration and 30 such sides are presented as a so-called cage. These clusters can be synthetically modified into various forms (open or closed) and can be substitued, for example by carbon atoms. This substitution can lead to the asymmetry of these molecules and thus to their chirality. Currently, their potential is also being studied in medicinal chemistry, where they could contribute to the design of new drugs. For instance, while they are been tested as HIV inhibitors, for boron neutron capture therapy, as CA IX inhibitors, they have also been suitable as part of hydrophobic pharmacophores in the design of drugs targetting the binding sites of various receptors. The task of this thesis is to test the influence of experimental conditions on the chiral separation of...
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HPLC Determination of Selected Antibiotics in Plasma
Sedláčková, Andrea ; Kastner, Petr (advisor) ; Pilařová, Pavla (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Andrea Sedláčková Supervisor: PharmDr. Petr Kastner, PhD. Title of thesis: HPLC Determination of Selected Antibiotics in Plasma The purpose of this diploma thesis was to develop and optimize conditions for determination of selected antibiotics (meropenem, imipenem, ceftazidime, piperacillin and linezolid) in plasma using high performance liquid chromatography with meropenem as the main substance to be determined. Separation was performed on the silica gel column Ascentis® C18 (100Å, 150 × 4,6 mm; 5 µm) using spectrofotometric detection. Selected antibiotics were detected at different wavelenghts (298 nm for meropenem and imipenem, 254 nm for ceftazidime and linezolid, 210 nm for piperacillin) according to maximum absorbance of the antibiotics. Different types of mobile phase were tested as well as different pH of buffer used for the mobile phase. The final mobile phase consisted of two components: mobile phase A: phosphate buffer 50 mM; pH 2,5 mobile phase B: acetonitrile Separation was achieved by gradient elution. The flow rate was set to 1 ml/min and the injection volume was 10 µl. The column oven was tempered to 25řC. Total runtime was 15 min. Validation of...
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The uset of HPLC in the field of chiral separations VII.
Štilcová, Kristýna ; Kučera, Radim (advisor) ; Pilařová, Pavla (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department: Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Student: Kristýna Štilcová Supervisor: doc. PharmDr. Radim Kučera, Ph.D. Consultant: Mgr. Ondřej Horáček Title of Thesis: The employment of HPLC in the field of chiral separations VII. Boron clusters are inorganic, synthetically prepared, three-dimensional, cage-like structures. Boron cluster compounds which are made only of boron and hydrogen atoms are completely symmetrical. Their symmetry can be disrupted by endo- or exoskeletal substitution resulting in chiral compounds. Carboranes, subgroup of the boron cluster compounds, contain at least one carbon atom in their structure and usually include exoskeletal substitution. Therefore, the metallacarboranes containing cobalt bis(dicarbollides) and 7,8-dicarba-nido-undecaborates that have been studied can be found among these chiral structures. Thanks to the specific properties such as high lipophilicity, metabolic stability and delocalized negative charge, the studied compounds can be used as isosteric substitution of phenyl ring in pharmacophores. Due to the growing interest in cobalt bis(dicarbollides) and 7,8-dicarba-nido-undecaborates and the importance of chirality in pharmacy, suitable conditions for chiral...
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Study of Retention Properties of Selected Tyrosine Metabolites under HPLC Conditions
Gieslerová, Michaela ; Kastner, Petr (advisor) ; Pilařová, Pavla (referee)
Charles University Faculty od Pharmacy in Hradec Králové Department od Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Michaela Gieslerová Supervisor: PharmDr. Petr Kastner, Ph.D. Title of thesis: Study of retention properties of selected tyrosine metabolites under HPLC conditions The aim of this diploma thesis was to study the retention properties of selected tyrosine metabolites under the high performance liquid chromatography using spectrofotometric (285 nm) and fluorimetric detection (emission 279 nm, excitation 320 nm). The total of eight analytes was examined - tyrosine, L-DOPA, dopamine, epinephrine, norepinephrine, homovanillic and vanillylmandelic acid. Firstly, we studied the effect of mobile phase composition, buffer concentration and pH for the separation of analytes. Analysis of selected analytes was performed using an KINETEX 5 μm EVO C18 column (150 mm x 3 mm, 5 μm; PHENOMENEX). The best separation showed the mobile phase methanol:100 mM acetic acid (pH adjusted with sodium hydroxide to 4,5); 5:95. Next, the effect of stationary phase for the separation of analytes was studies with the mobile phase described above. A total of 7 different columns were studied, namely stationary phases with modified silica gel C18 group, PFP group, combined stationary phase (C18 group...
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HPLC analysis of podophyllotoxin
Nováková, Markéta ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Markéta Nováková Tutor: PharmDr. Pavla Pilařová, Ph.D. Title of Thesis: HPLC Analysis of Podophyllotoxin The diploma thesis is focused on optimalization of HPLC method for the analysis of podophyllotoxin. The method was developed for the analysis of Juniperus virginiana leaves extract. Various stationary phases, mobile phases, temperatures, ultrasonic extraction times were tested. Following conditions were chosen as optimal: 30 minutes ultrasonic extraction time, LiChrospher 100 RP-18 250-4 column, 0,1% CH3COOH:ACN 45:55 mobile phase, temperature 40 řC, flow rate 0,8 ml/min, sample volume 3 μl, fluorescence detection λex 240 nm, λem 320 nm, time of analysis 9 minutes. The method was also developed for the analysis of plant tissue cultures of Juniperus virginiana, for which gradient elution was chosen. This method had following conditions: LiChrospher 100 RP-18 250-4 column, mobile phase 0,1% CH3COOH:ACN, change of acetic acid ratio: 0-8 min 70-10 %, 8-9 min 10 %, 9-10 min 10- 70 %, 10-12 min 70 %, temperature 40 řC, flow rate 0,8 ml/min, sample volume 3 μl, fluorescence detection λex 240 nm, λem 320 nm, time of analysis 12 minutes. Subsequently, both methods were...
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The uset of HPLC in the field of chiral separations VII.
Štilcová, Kristýna ; Kučera, Radim (advisor) ; Pilařová, Pavla (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department: Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Student: Kristýna Štilcová Supervisor: doc. PharmDr. Radim Kučera, Ph.D. Consultant: Mgr. Ondřej Horáček Title of Thesis: The employment of HPLC in the field of chiral separations VII. Boron clusters are inorganic, synthetically prepared, three-dimensional, cage-like structures. Boron cluster compounds which are made only of boron and hydrogen atoms are completely symmetrical. Their symmetry can be disrupted by endo- or exoskeletal substitution resulting in chiral compounds. Carboranes, subgroup of the boron cluster compounds, contain at least one carbon atom in their structure and usually include exoskeletal substitution. Therefore, the metallacarboranes containing cobalt bis(dicarbollides) and 7,8-dicarba-nido-undecaborates that have been studied can be found among these chiral structures. Thanks to the specific properties such as high lipophilicity, metabolic stability and delocalized negative charge, the studied compounds can be used as isosteric substitution of phenyl ring in pharmacophores. Due to the growing interest in cobalt bis(dicarbollides) and 7,8-dicarba-nido-undecaborates and the importance of chirality in pharmacy, suitable conditions for chiral...
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