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Solid phase extraction and its miniaturization by method Lab-On-Valve
Krchňáková, Eva ; Chocholouš, Petr (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Bc. Eva Krchňáková Supervisor: PharmDr. Petr Chocholouš, Ph.D. Title of diploma thesis: Solid phase extraction and its miniaturization by method Lab-On- Valve. This thesis deals with the ability to automate solid phase extraction (SPE), in connection with the bead injection (BI). The aim was to test the replacement of microparticles in the bead injection with sorbent from SPE cartridges, determinate the behavior of sorbent in this system and then determine whether it is possible to miniaturize all the steps of solid phase extraction that are necessary to implement the method. In the work was done manual solid phase extraction, where were tested seven sorbents: Lichrolut Merck RP-18, DSC-18 DSC-18Lt, DSC-8, PH-DSC, DSC-CN and Oasis HLB. The experiment resulted in the best possible conditions for carrying out solid phase extraction as follows: activation of the sorbent and elution of analyte 100% acetonitrile, solvation of the sorbent, the dissolution of vitamin D and ballast from the sorbent washed with dilute acetic acid of pH 3. Oasis HLB from Waters Company is the most suitable sorbent for the system, which is water-wettable and should not be dispersed by the system, which was...
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Solid phase extraction and its miniaturization by method Lab-On-Valve
Krchňáková, Eva ; Chocholouš, Petr (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical chemistry Candidate: Bc. Eva Krchňáková Supervisor: PharmDr. Petr Chocholouš, Ph.D. Title of diploma thesis: Solid phase extraction and its miniaturization by method Lab-On- Valve. This thesis deals with the ability to automate solid phase extraction (SPE), in connection with the bead injection (BI). The aim was to test the replacement of microparticles in the bead injection with sorbent from SPE cartridges, determinate the behavior of sorbent in this system and then determine whether it is possible to miniaturize all the steps of solid phase extraction that are necessary to implement the method. In the work was done manual solid phase extraction, where were tested seven sorbents: Lichrolut Merck RP-18, DSC-18 DSC-18Lt, DSC-8, PH-DSC, DSC-CN and Oasis HLB. The experiment resulted in the best possible conditions for carrying out solid phase extraction as follows: activation of the sorbent and elution of analyte 100% acetonitrile, solvation of the sorbent, the dissolution of vitamin D and ballast from the sorbent washed with dilute acetic acid of pH 3. Oasis HLB from Waters Company is the most suitable sorbent for the system, which is water-wettable and should not be dispersed by the system, which was...
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