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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Srovnávací analýza působení exogenního působení cytokininů s inhibicí degradace jejich endogenních hladin u Arabidopsis thaliana
 Koukalová, Vladěna
Agricultural production is negatively affected by stress factors like drought or rising ambient temperature. Genetic engineering and production of specific GMO seems to be very promising in protection of the crop yield however these are strictly limited for the use in the field. For this reason alternative approaches like application of growth regulators are in the focus of recent research. INCYDE is a new potent growth regulator discovered as inhibitor of cytokinin degradation. In this Diploma thesis we show that high levels of INCYDE could also activate AHK4, cytokinin receptor, and act like cytokinins. Comparison of trans-zeatin and INCYDE showed that application of these regulators results in spatial-specific pattern in activation of the cytokinin signaling. trans-zeatin and INCYDE also induce different changes in proteom mainly in proteasome pathway, gluconeogenesis and fatty acids metabolism. Application of INCYDE simultaneously with inducing drought stress revealed that INCYDE could decrease sensitivity to this stressor. This feature seems to be very perspective for its utilization in the agriculture.
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Vliv modulace hladin cytokininů na fenom a proteom rostlin
 Koukalová, Vladěna
Negative effects of biotic and abiotic stressors of the environment are limiting factors for agricultural production. To minimize the effect of these factors, molecular genetics focuses on preparing the new genetically modified organisms with higher tolerance to stress. However, recent legislation strongly limits the use of GMO and thus new approaches focused on similar outcomes are followed. One of these approaches is developing of new pharmaceuticals with positive effects on plant tolerance to environmental stressors. This thesis is focused on the characterization of such substance called INCYDE. INCYDE inhibits degradation of plant hormons cytokinins, which are known to have positive effects on delaying senescence and tolerance to extreme drought conditions. In the classical bioassay based on inhibition of the main root growth, INCYDE was shown to be active as one of the most active cytokinin trans-zeatin. Analysis of the lines with modified canonical cytokinin signalling pathway showed INCYDE and trans-zeatin to act by same signalling components. Despite the fact of similar effects on morphological alterations, proteom analysis revealed differences in the effect of INCYDE and trans-zeatin on protein abundance. Differences on the proteom level will be analyzed in following study.
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See also: similar author names
2 Koukalová, Veronika
1 Koukalová, Vlasta
2 Koukalová, Věra
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