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Analysis of flagellar proteins in Clostridium difficile isolates of clinically relevant PCR-ribotypes Houdková, Kateřina ; Konečná, Klára (advisor) ; Tichý, Aleš (referee) Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Kateřina Houdková MPharm Supervisor: RNDr. Klára Konečná, Ph.D. Consultant: PharmDr. Jiří Dresler, Ph.D. Title of rigorous thesis: Analysis of flagellar proteins in C. difficile isolates of clinically relevant PCR-ribotypes Background: Strains of C. difficile of known human epidemiologic importance are associated with severe clinical features of C. difficile infection (CDI). In this study, a panel of eight different PCR-ribotypes (RTs) with their proteins released in vitro were subjected to analysis. The aim of this work is to monitor the relationship between secretions of individual proteins associated with flagellar formation and function in C. difficile strains of variable virulence. Methods: Within our research, a combination of tandem mass spectrometry with liquid chromatography was used. The semi-quantitative analysis employed label free quantification (LFQ) approach. Results: From the quantifiable proteins, 17 were significantly increased in functional annotations. Among them, several known factors connected with flagellar assembly and other functions were identified. Higher expression of selected flagellar proteins clearly distinguished RTs 027, 176, 005 and 012, confirming the pathogenic... Detailed record

Prevence of youth people unemployment in regional contexts. HOUDKOVÁ, Kateřina All industrial countries face the problem of unemployment. High percentage of unemployed has negative influence on social situation, state economy, and also quality of life. Decreasing unemployment is long term goal of each particular government. Most emphasis is given to mitigation of unemployment of people aged over 50 years, under 25 years and long-term unemployment. Important part of unemployed in all czech regions consists of graduates and youngsters. Graduates are impaired by the lack of practice and necessary occupational habits. Youngsters usually have problems with their lack of qualification and legislative obstacles. Inability to find a job grows feelings of inconclusiveness, vulnerability and inferiority among young people which leads them to idleness and socially not beneficial activities. Main goal of this work is to evaluate different solutions of young people unemployment problem in regions of Plzeň and České Budějovice; to compare unemployment percentage in these regions and define typical patterns in which the problem appears. I collected quantifiable field data using a questionnaire. As a second method of research I used semi-standardized interview with directors of technical schools. Third method used is secondary data analysis. Most important roles in prevention of the unemployment of youngsters are family background and rising to career choice in elementary school. Also system of guidance services is very important. Then motivation for higher education and prevention of early school leaves plays important role. Employment office can help young people by using the tools of active employment policy. The unemployment in the region of České Budějovice is higher than in the region of Plzeň. Both regions share good opportunities in technical professions. It appears to be beneficial to increase demand for study of technical branches among pupils. Detailed record

See also: similar author names
2	HOUDKOVÁ, Kateřina
3	HOUDKOVÁ, Klára
1	Houdková, Kamila
1	Houdková, Karina
3	Houdková, Klára
1	Houdková, Kristine
1	Houdková, Kristýna

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