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Comparison of the species spectrum of spruce bark beetle pathogens and its predators from genus Thanasimus caught in pheromone traps
Šídová, Michaela ; Lukášová, Karolína (advisor) ; Holuša, Jaroslav (referee)
Spruce bark beetle (Ips typographus (Linnaeus, 1758)) is our most serious pests, frequently invading spruce stands. The most important predators of I. typographus are two species from genus Thanasimus - Thanasimus formicarius (Linnaeus, 1758) and Thanasimus femoralis (Zetterstedt, 1828). Monitoring of these three species were carried out from April to August, using pheromone traps type Theyson with pheromone lures designed for their capture. New pheromone lure attracting beetles of genus Thanasimus has been tested. It was used a total of six traps that were placed at three locations. Two traps were always placed at one location, one with pheromone lure Pheroprax attracting spruce bark beetles and the second one with pheromone lure called Thanasiwit attracting beetles of the genus Thanasimus. All traps were checked once a week. A total of 10 291 of spruce bark beetles were captured, 25 specimens of T. formicarius and 7 individuals of T. femoralis. Spruce bark beetle flight activity had two generations and one sister brood. The first catches were recorded on 26 April 2015 and the end of the flight activity was recorded on 26 July 2015. The species T. formicarius flight activity was from 12 April 2015 to 2 August 2015 and during this period two generations were recorded. For T. femoralis flight activity was from 17 April 2015 until 9 August 2015, during this period we also recorded two generations, which took place before and after the flight activity of I. typographus. Laboratory work was conducted after the end of captures. From every of sample twenty individuals were dissected and then inspected using a light microscope to presence of pathogens. The most frequently identified pathogen of spruce bark beetles were nematodes, but only in one case nematods were identified in T. formicarius. Other pathogens were found only in the spruce bark beetles and they included microsporidia Chytridiopsis typographi, neogregarine Mattesia schwenkei and nematodes of the genus Contortylechus sp.

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3 Šídová, Markéta
3 Šídová, Monika
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