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Proteom rostlin v reakci na abiotický stres
Čotková, Veronika
Plants as a sessile organisms have evolved complex mechanisms to cope with unfavorable environmental conditions. This diploma thesis summarizes current state of knowledge and focuses on light, temperature and phytohormones in plant abiotic stress responses. The practical part is devoted to proteomics and completes and extends the project started during my Bachelor program. Transgenic Arabidopsis seedlings carrying inducible barley cytokinin oxidase/dehydrogenase (CaMV35S> GR> HvCKX2) were profiled to elucidate proteome-wide responses to down-regulation of cytokinin levels under heat stress at standard (80 umol.m-2.s-1) and low light intensity (20 umol.m-2.s-1). In total, 2-DE analysis and mass spectrometry analysis revealed 63 differentially abundant proteins involved in diverse metabolic processes. These data provide evidence of a link between temperature, light and cytokinin signaling in Arabidopsis and will be used in the modeling of cytokinin-light-temperature interactions.
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Performance and Sexuality at fictions by Jakuba Katalpa
Čotková, Veronika ; Heczková, Libuše (advisor) ; Vojvodík, Josef (referee)
The subject of this thesis is to find and evaluate performative acts of the characters at proses written by Jakuba Katalpa. Since it is impossible to capture performative acts of all social discourses, we will focus only on the discourse of gender. Especially, because Jakuba Katalpa sees the world in a way that rise the question of a gender and feminism. Mainly we will focus on performative acts of characters and of a narrator at Katalpa's proses Mrtvé moře and Je hlína k snědku? Firstly, we attempt to define basic concepts such as a gender, an identity, and a performance and we will try to outline the basic theory on which we rely. We will also try to determine how, at theese proses, is an identity unregistered, how and by using which acts are characters identified with gender models, and how they differ from them. The result of the work would be to find and evaluate common principle of performance and its potential to change traditional stereotypes.
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Performance and Sexuality at fictions by Jakuba Katalpa
Čotková, Veronika ; Heczková, Libuše (advisor) ; Vojvodík, Josef (referee)
The subject of this thesis is to find and evaluate performative acts of the characters at proses written by Jakuba Katalpa. Since it is impossible to capture performative acts of all social discourses, we will focus only on the discourse of gender. Especially, because Jakuba Katalpa sees the world in a way that rise the question of a gender and feminism. Mainly we will focus on performative acts of characters and of a narrator at Katalpa's proses Mrtvé moře and Je hlína k snědku? Firstly, we attempt to define basic concepts such as a gender, an identity, and a performance and we will try to outline the basic theory on which we rely. We will also try to determine how, at theese proses, is an identity unregistered, how and by using which acts are characters identified with gender models, and how they differ from them. The result of the work would be to find and evaluate common principle of performance and its potential to change traditional stereotypes.
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Proteom rostlin v reakci na abiotický stres
Čotková, Veronika
Plants as a sessile organisms have evolved complex mechanisms to cope with unfavorable environmental conditions. This diploma thesis summarizes current state of knowledge and focuses on light, temperature and phytohormones in plant abiotic stress responses. The practical part is devoted to proteomics and completes and extends the project started during my Bachelor program. Transgenic Arabidopsis seedlings carrying inducible barley cytokinin oxidase/dehydrogenase (CaMV35S> GR> HvCKX2) were profiled to elucidate proteome-wide responses to down-regulation of cytokinin levels under heat stress at standard (80 umol.m-2.s-1) and low light intensity (20 umol.m-2.s-1). In total, 2-DE analysis and mass spectrometry analysis revealed 63 differentially abundant proteins involved in diverse metabolic processes. These data provide evidence of a link between temperature, light and cytokinin signaling in Arabidopsis and will be used in the modeling of cytokinin-light-temperature interactions.
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