Rigorous theses

Rigorous theses 7,828 records found  previous11 - 20nextend  jump to record: Search took 0.00 seconds. 
Binding opinions as an instrument of the natural and landscape protection
Štěpánková, Jana ; Franková, Martina (advisor) ; Pokorný, Jiří (referee)
This thesis focuses on the binding opinion and defines its key role in nature and landscape protection. From the author's viewpoint construction activity is an activity with the greatest impact on nature and landscape and the submitted work focuses, among other things, on the evidence that the purpose of issuing binding opinions is the regulation of construction. The thesis consists of introduction, conclusion and five chapters divided into subchapters according to logical and well-arranged structure. The first chapter discusses the role of the body concerned in the protection of the public interest, which is the protection of nature and landscape. The second chapter is structured into several subchapters and is devoted to the description of the binding opinion. The author analyzed its definition features, its legal nature and its content requirements. The last part of this chapter is devoted to the process of issuing binding opinions. Furthermore, the author focused on the review mechanism of binding opinions, namely its legislation in the Code of Administrative Procedure, the Code of Administrative Justice, including a deviation from the general regime laid down by the Building Act. The fourth chapter is devoted to the definition of the involved authorities concerned by the law on nature and...
The study of properties of radiolabelled monoclonal antibodies for imaging in oncology
Janoušek, Jiří ; Trejtnar, František (advisor) ; Kozempel, Ján (referee) ; Lázníčková, Alice (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Jiří Janoušek, MSc. Supervisor: Assoc. Prof. PharmDr. František Trejtnar, CSc. Consultant: Mgr. Pavel Bárta, Ph.D. Title of doctoral thesis: The study of properties of radiolabelled monoclonal antibodies for imaging in oncology Monoclonal antibodies belong to the group of targeted biological drugs that are currently successfully applied in various medical specialties, including oncology. The ability to specifically bind to antigens present in a tumour tissue is their significant attribute that can be used for imaging tumours in Nuclear Medicine. Receptors for vascular endothelial growth factor (VEGFR) and epidermal growth factor (EGFR) are possible tumour specific targets that are characteristic for some types of proliferating tumour tissue. The attachment of appropriate radionuclide is necessary for the potential radiodiagnostic use of monoclonal antibody. This task requires chemical modification of the antibody molecule that can damage its immunoreactivity and the binding to the target receptor. The aim of the doctoral thesis was to study the influence of labelling conditions with selected diagnostic radionuclides on radiochemical characteristics, in vitro receptor specific binding and...
Development of novel cholinesterase modulators
Górecki, Lukáš ; Korábečný, Jan (advisor) ; Mičuda, Stanislav (referee) ; Roh, Jaroslav (referee)
Tittle: Development of novel cholinesterase modulators Author: Mgr. Lukáš Górecki Abstract The enzyme acetylcholinesterase (AChE) is a key component in cholinergic synapses and at the neuromuscular junctions. Its physiological function is essential in humans, as well as in animal species, including insects. In pathological conditions, AChE is also involved in various disorders such as Alzheimer's disease (AD) or myasthenia gravis. The significance of the enzyme has also been demonstrated by its successful use as a target in insecticides. In addition, it is the key mediator in the manifestation of symptoms of intoxication after exposure to Chemical warfare agents (CWA) (Nerve agents; NA). Herein, I report my study of three different aspects of this enzyme: as an anti-AD target; as an insecticidal target; and as an enzyme to be reactivated after intoxication by organophosphorus compounds (OPCs). Since the introduction of tacrine in 1993 as an anti-AD drug, much attention has been paid to the development of novel AChE inhibitors; some, such as donepezil, have been successfully marketed. At present, there is still a need for efficient drugs against AD. As AD is a multifactorial disorder, it believed that it cannot be treated by simply targeting one pathological condition. Therefore, the so-called multi-target...
A study of interactions of novel anticancer drugs with drug transporters.
Šorf, Aleš ; Čečková, Martina (advisor) ; Kollár, Peter (referee) ; Froňková, Eva (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Mgr. Aleš Šorf Supervisor: doc. PharmDr. Martina Čečková, Ph.D. Title of doctoral thesis: A study of interactions of novel anticancer drugs with drug transporters. Despite the significant progress in recent years, the efficiency of anticancer therapy is still complicated by drug resistance. This phenomenon can be mediated by pharmacokinetic mechanisms such as efflux via ATP-binding cassette (ABC) transporters and biodegradation of parent drug to inactive metabolites via metabolizing enzymes. These proteins also play a key role in absorption, distribution and excretion of drugs. When two or more interacting substances are administered simultaneously, the pharmacokinetic drug-drug interactions may occur. Since the targeted protein kinase inhibitors are becoming more common and important component of modern anticancer therapy, the aim of this study was to elucidate interactions of selected drugs of this novel therapeutic group with ABC transporters and also drug metabolizing enzymes by in vitro approaches. We further examined relevant substances ex vivo using mononuclear cells isolated directly from the blood of patients de novo diagnosed for acute myeloid leukemia (AML). Regarding the...
Legal regulation of content of B2C contracts (from the perspective of consumer protection)
Krahulíková, Kateřina ; Josková, Lucie (referee)
This diploma thesis deals with the content of the consumer contract and its regulation from the perspective of consumers. The aim of this thesis is to analyse this particular regulation, decide whether it is sufficient, where can be seen some deficiencies and whether the regulations fulfil its function, that is to protect the weaker party. The thesis is divided into five fundamental parts. The first one is concerned with the introductory issues, such as the consumer itself, consumer protection, why is the consumer actually protected, the principles on which is the protection based. The second part looks into the unfair terms in consumer contracts. The emphasis is placed on problematic provisions with respect to the Court of Justice case-law as well as the national case-law. An independent head is devoted to the possibility of the contractual penalty in terms of trade. The third section deals with contractual information requirements. It summarizes the European regulation. It also compares the preceding regulation with the new one in order to find the current tendencies. The contractual information requirements are viewed critically, based on specialized comments and analysis. The national regulation is divided into the general regulation and consumer regulation considering the difference between...
The iron disposition regulates liver homeostasis of cholesterol and bile acids
Prašnická, Alena ; Mičuda, Stanislav (advisor) ; Pejchal, Jaroslav (referee) ; Večeřa, Rostislav (referee)
Changes in the iron content are among the most common metabolic diseases with a number of possible pathophysiological consequences for various body functions. The phenomenon accompanying these situations is the change in cholesterol homeostasis with possible extension to bile acids (BA), ie major executive and regulatory molecules in the metabolism of endobiotics, what can play an important role in associated organ dysfunctions. Since there was only a minimal amount of information in this area, the aim of this work was to evaluate the effect of iron overload and deficiency on the processes involved in BA turnover with the identification of involved molecular mechanisms. The first assessed field was the evaluation of iron deficiency (ID) using a suitable rat model, where ID was induced by a specific diet. This has demonstrated a significant inducing effect on both classical and alternative pathways for BA synthesis from cholesterol. Together with the induction of cholesterol biliary secretion in ID group it led to statistically significant decrease of plasma cholesterol concentrations. The subsequent BA-dependent choleretic effect was induced by their increased liver disposition, without affecting the expression of corresponding transporters, as verified by the kinetic study with 3 H-taurocholate....
Unilateral set-off
Veselý, Martin ; Thöndel, Alexandr (referee)
The subject of the diploma thesis is the legal institute of one-sided credit and comparison of its adjustment from several levels; comparison of legislation in the Civil and Commercial Code, comparison with the Czech version, which in 2012, after the recodification of private law, underwent a fundamental change, thus deviating from the modification until then identical with the Slovak Republic and last but not least with the proposal of a common frame of reference as a unifying platforms for the convergence of the private law adjustments of individual Member States of the European Union. The aim of this work is not only to describe the differences in the legislative approach to adapt this institute across the above mentioned levels, a description of the evolution of this legal regulation, but also the definition of lege de ferenda. The work points to the need for a change in the adaptation of this institute; in particular, the need for a clear definition of the conditions for offsetting the outstanding claim, including the moment of its effect. Only in this way will the legal regulation more converge with the requirements of unifying the platform of the common frame of reference, thus eliminating current interpretative discrepancies or imperfections in the case law. In the lege de ferenda...
Nukleární receptory - studium nových ligandů a význam genové variability
Carazo Fernández, Alejandro ; Pávek, Petr (advisor) ; Mičuda, Stanislav (referee) ; Vrzal, Radim (referee)
IN ENGLISH LANGUAGE Candidate: Mgr. Alejandro Carazo Fernández Supervisor: Prof. PharmDr. Petr Pávek, PhD. Title of the doctoral thesis: Nuclear receptors - new ligands study and importance of the genetic variability Nuclear receptors (NRs) constitute a superfamily of transcription factors, which regulate the expression of target genes upon the binding of a ligand. These receptors can be classified in steroid receptors, "orphan receptors" and "adopted orphan receptors" depending on the affinity to an endogenous ligand. Nuclear receptors play important roles in physiological processes and are widely distributed in the human body. Thus, adipogenesis, lipolysis, insulin sensitivity, oxidative metabolism, fatty acid homeostasis, cholesterol homeostasis, gluconeogenesis, glycogen homeostasis, triglyceride metabolism among other processes, are regulated by nuclear receptors. During my study, we have tested several sets of drugs, endogenous, natural and synthetic, in several nuclear receptors, focusing mainly on constitutive androstane receptor (CAR) and to a lesser extent on pregnane X receptor (PXR). My main aim was to find a new and reliable ligand or activator for human CAR. In addition, I aimed to study the mechanism of action by which these compounds interact with the receptor and how they trigger...
Analysis of drug-related problems in a health facility IV.
Šubrtová, Linda ; Malý, Josef (advisor) ; Zimčíková, Eva (referee)
Analysis of drug-related problems in a health facility IV. Author: Linda Šubrtová Tutor: PharmDr. Josef Malý, Ph.D. Department of Social and Clinical Pharmacy, Faculty of Pharmacy in Hradec Králové, Charles University Introduction: Increasing use of drugs leads to a growing risk of occurence of drug-related problems. These problems can often endanger patient's health and increase the economic cost of the treatment. However, many of these problems can be prevented by controls and collaboration of all health care subjects. Objective: The aim of this work was to carry out a revision of pharmacotherapy in a rehabilitation health facility and to analyse acquired drug-related problems. Methodology: The collection of data took place from 13 to 15 February 2017 in health facility in the Czech Republic. The review of pharmacotherapy was performed by a three-member team (clinical pharmacist, pharmacist, work researcher). The data obtained from the provided medical records were recorded into the database, which was prepared for the purpose of the pharmacotherapy review. Recorded were: the patient's age and sex, allergic, family, work, social and personal history, physical and laboratory examinations and complete pharmacotherapy, which included over-the-counter drugs and dietary supplements, including dosage...
The influence of polyphenolic compounds and their metabolites on platelet activation
Applová, Lenka ; Mladěnka, Přemysl (advisor) ; Bultas, Jan (referee) ; Šimůnek, Tomáš (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Applová Lenka, MSc. Supervisor: Assoc. Prof. Přemysl Mladěnka, PharmD., Ph.D. Title of Doctoral Thesis: The influence of polyphenolic compounds and their metabolites on platelet activation Blood aggregation is a very difficult and complex process, in which platelets play crucial role. On the other hand, their activation is excessive and therefore undesirable under pathophysiological conditions. Cardiovascular diseases are the most common cause of mortality in developed countries and unfortunately, despite significant advances in treatment, the Czech Republic remains in mortality from these diseases behind most Western European countries. Epidemiological studies suggest, that higher intake of (iso)flavonoids is associated with reduced cardiovascular mortality. It is also worth mentioning, that the Czech Republic is among European countries with the lowest content of flavonoids in diet. The aim of this doctoral thesis was to find out, if polyphenolic compounds, especially (iso)flavonoids and/or their metabolites, are able to influence platelet aggregation and thus indirectly to support epidemiological findings, i.e. whether there may be a link between these substances and possible positive...

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