Methods

Methods 640 records found  previous11 - 20nextend  jump to record: Search took 0.00 seconds. 
Postup dezinfekce a čištění textilních závěsů pečetí
Drábková, Klára ; Škrdlantová, Markéta ; Nagyová, Dominika ; Krejčí, Jan ; Bacílková, Bronislava ; Bureš Víchová, Jana ; Ďurovič, Michal
Textilní závěsy jsou nedílnou součástí archivních dokumentů s přivěšenou pečetí a jejich udržení v dobrém stavu je podmínkou jak pro uchování pečetě, tak i právní hodnoty listiny. Proto je cílem této metodiky doporučit vhodný a bezpečný postup dezinfekce a čištění textilních závěsů pečetí. Konzervování textilních závěsů je specifické kvůli jejich kontaktu s dalšími materiály archiválií (pečeť, papír, pergamen apod.) a není tedy možné využít všechny metody běžně užívané v procesu konzervování-restaurování textilií. Přínosem této metodiky je zohlednění výše uvedených specifik. Textilní závěsy pečetí, mohou být znečištěny jak prachovými nečistotami, tak nečistotami vzniklými při výrobě pečetí, či předchozím restaurátorským zásahem, případně mohou být mikrobiologicky napadeny. Vhodná dezinfekce a dostatečné vyčištění textilního závěsu přispějí k jeho dlouhodobé stabilitě.
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Certified methodology for rapid evaluation of the current level of resistance of stored product infesting pests to phosphine gas based fumigants: Methodology for DDD, agriculture and food industry workers
Aulický, Radek ; Stejskal, Václav ; Frýdová, Barbora
The presented new methodology is a set of information and procedures for evaluation of the sensitivity/resistance of storage pests to the phosphine (PH3) which is plant protections and/or biocide active substance commonly used for fumigation of infested commodities and empty store spaces as a key pest control intervention. The procedure is based on the international tests Phosphine Resistance Test Kit (Detia DEGESH's) and on the originally scientifically developed findings and information. The regular assessment of the resistance or resistance of field populations is one of the pillars of IPM whose implementation into practice helps to reduce the consumption of chemical products.
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Methodics of detection of three cereal viruses (BYDV, WDV, WSMV) by Multiplex TaqMan RT-qPCR
Jarošová, Jana ; Kumar, Jiban
The family Poaceae is one of the most important groups of plants in the terms of agricultural production. The family Poaceae also hosts many pathogens including >100 known virus species. The three most important viruses affecting cereals in the Czech Republic are Barley yellow dwarf virus (BYDV), Wheat dwarf virus (WDV) and Wheat streak mosaic virus (WSMV). In this methodology we provide an assay for multiplex detection and quantification of those viruses by TaqMan RT-qPCR. The methodology is meant to serve scientific workers as well as the employees of state laboratories as a tool for individual virus occurrence monitoring in the Czech Republic.
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Resynthesis of oilseed rape from Brassica rapa and Brassica oleracea
Klíma, Miroslav ; Hilgert-Delgado, Alois ; Ulvrová, Tereza ; Bryxová, Pavla ; Vítámvás, Pavel ; Prášil, Ilja Tom ; Kosová, Klára
In order to extend the variability of the genofond, so-called resynthesis can be used, when new genotypes are created by crossing selected Brassica rapa and B. oleracea, by early isolation of immature embryos from young eggs and their in vitro fertilization (Hilgert-Delgado et al., 2015). It has been shown that resyntetized genotypes are distinct from rape materials grown at present (Sosnowska et al., 2010), so that they can serve as a source of new variability in breeding programs focusing on cytoplasmic and pollen sterility, resistance to pathogens and tolerance to stresses (Warwick et al., 2009). Representatives of the initial components are characterized by wide variability in terms of morphology and agronomic characteristics, and are a prospective source of heterosis and other traits (Wu et al. The use of resyntetized genotypes for the direct production of rapeseed varieties is not possible due to the poor quality of the seed of these materials. It is mainly the high content of glucosinolates and erucic acid, typical of most of the initial components (Jesske et al., 2013). However, the required properties can be transferred by crossing to conventional breeding materials (Hilgert-Delgado et al., 2017).
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Determination of the frost tolerance of rapeseed varieties in early stages of plant development
Prášil, Ilja Tom ; Klíma, Miroslav ; Musilová, Jana ; Kosová, Klára ; Vítámvás, Pavel
The methodology is focused on the determination of frost tolerance of varieties, breeding materials and gene sources of rapeseed in the early stages of plant development using a direct frost test. Rapeseed plants grow in pots located outdoors under natural conditions. The sowing of germinated rapeseed seeds is carried out during October so that until the end of November the plants reach the first leaf stage. The plants are exposed to a frost test in pots from December to February according to the described procedures.
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Methodology for long-term evaluation of transgenic plum cv. ´HoneySweet´ resistance against Plum pox virus: Certified methodology applied
Polák, Jaroslav ; Komínek, Petr ; Krška, Boris ; Jarošová, Jana
Methodology describes procedure of establishing the field trial with Biotech plum cv. ´HoneySweet´, growing the plants in the experimental orchard and evaluation of plum resistance against Plum pox virus infection, as well as against co-infection with other two viruses, Apple chlorotic leafspot virus and Prune dwarf virus.
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Farming practices for efficient use of nitrogen and reduction of its losses: Certified methodology for practice
Klír, Jan ; Haberle, Jan ; Růžek, Pavel ; Šimon, Tomáš ; Svoboda, Pavel
The methodology describes the main risks of nitrogen losses and recommended practices for their reduction. Practically applicable methods for the evaluation of the nitrate leaching risk depending on the soil, crop, root range, croping system, irrigation and weather conditions are described in detail. Consequently, suitable soil treatments and fertilization are proposed to improve the utilization of the nitrogen and uniformity of the water soaking.
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Methodology for detection identification of fungi responsible for the cereal contamination with ochratoxin A and citrinin using PCR: Methodology for practice
Salava, Jaroslav ; Novotný, David
The aim of this methodology was to describe a protocol for the rapid and reliable detection of microscopic fungi from the genusPenicillium and Aspergillus capable of producing mycotoxins ochratoxin A and citrinin in cereals, preferably prior to their production and accumulation, using polymerase chain reaction (PCR) and their use, e.g. for the evaluation of health and hygienic safety of plant products.
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Short-term rainfall for hydrological modeling and design of small water management structures in the landscape
Kavka, P. ; Müller, Miloslav ; Strouhal, L. ; Kašpar, Marek ; Bližňák, Vojtěch ; Landa, M. ; Weyskrabová, L. ; Pavel, M. ; Dostál, T.
The aim of this methodology is to reduce the current knowledge deficit in the field of short-term rainfall variability and its application in hydrological modeling and design of small water structures and conservation measures within the catchment. The methodology defines six generalized short-term rainfall scenarios with specific temporal distribution of rainfall intensity. Next their variability related to their occurence frequency and spatial distribution accross Czech Republic is presented. These scenarios were derived from the 10-years time series of radar rainfall measurements adjusted with the daily totals from ground gauging network.

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