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Methodology for co-cryostorage of virus isolates in their host plants and relevant data
Bilavčík, A. ; Bjerkestrand, R. ; Bjelland, B. ; Blystad, D.-R. ; Bobrova, O. ; Fránová, Jana ; Faltus, M. ; Hamborg, Z. ; Hammond Hammond, S. D. ; Koloniuk, Igor ; Zámečník, J.
Currently, the nursery production of plant material emphasizes the production of healthy, virus-free material. For this reason, it is necessary to have reference plant materials in which the virus is present. Some types of viruses are difficult to maintain outside the plant host. Because of the potential transmission to outdoor plantings, it is necessary to keep infected plants in special cultivation areas, and net-houses, which brings considerable financial and labour costs. Therefore, a methodology was developed in which the virus status in plant material is analysed, the infected material can be stored using cryopreservation in liquid nitrogen and then regenerated in vitro, and relevant data in GeneEver cryobox can be stored.
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Virus eradication method by cryopreservation tool - a cryoknife
Bilavčík, A. ; Bobrova, O. ; Faltus, M. ; Fránová, Jana ; Hammond, S. ; Koloniuk, Igor ; Zámečník, J.
Currently, in the nursery production of plant material, emphasis is placed on the propagation of healthy material. For this reason, it is necessary to pay attention to the topic of healthy virus-free nuclear stock when preserving collections of genetic resources of vegetatively propagated plants. Some raspberry viruses are difficult to remove from the plant host by existing methods of thermotherapy or chemotherapy. That is why, using the example of black raspberry necrosis virus (BRNV), a comprehensive methodology for testing health status and cryotherapy in raspberry was developed. The resulting methodical procedure makes it possible to eradicate BRNV from raspberry using cryotherapy of in vitro cultures. The user of the methodology is the Ministry of Agriculture, which applies it as part of the 'National Program for the Conservation and Use of Genetic Resources of Plants, Animals and Microorganisms Important for Nutrition and Agriculture'.
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Supporting grassland biodiversity through small-scale fallows
Fabšičová, Martina ; Frei, I. ; Jiroušek, M. ; Smetanová, S. ; Šipoš, Jan ; Trnka, F. ; Vymyslický, T. ; Winkler, J. ; Zdražílková, M.
The main goal of the methodology presented here is to propose a system of recommendations for applying fallow in nature conservation based on our experiments. Due to the independence of agricultural land outside specially protected areas with direct payments from the Ministry of Agriculture, it is currently important to apply the methodology, especially in large protected areas where the primary objective is to preserve natural and cultural heritage. Annual and perennial fallows, i.e. biotopes adapted toregular soil disturbance, offer suitable habitats for a number of now-threatened plant and animal species. This is a technically and economically easy solution, but it is challenging to select a suitable location to achieve maximum efficiency. Ploughing must not be used in species-rich grasslands which would lead to ruderalization and degradation of the native vegetation. Furthermore, it must not be carried out on land at risk of erosion. Linear grassland margins, which have been ploughed in the past, recently grassed fields, fallows, and field margins should be selected for fallow management. Nearby populations of invasive plant species can be a problem. Our recommendations for the use of fallow management relate primarily to land in lowland and upland areas with a lower soil nutrient supply.
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Fallow management on grasslands and pastures
Fabšičová, Martina ; Frei, I. ; Jiroušek, M. ; Smetanová, S. ; Šipoš, Jan ; Trnka, F. ; Vymyslický, T. ; Winkler, J. ; Zdražílková, M.
The presented methodology summarizes the results of long-term research of fallows and provides important information for a large spectrum of users. We present here thepossibilities, of how small-scale fallow management can practically adapt into the current meadow and pasture management system. The methodology is aimed primarily at theagricultural public. The users will find here the tested methodological procedures and recommendations for practical inclusion of fallows into the management plans. It isrecommended here to create fallows on the edges of meadows and pastures adjacent to other habitats, such as steppes, balks, bushes, forests, or arable land. Fallows then play the role of ecotones, i.e. transitional habitats. The greatest diversity of both plant and animal species is generally concentrated in such habitats. Fallows enable the survival, restoration, and strengthening of populations of rare and endangered plant species, not only weeds. Furthermore, fallows will support the occurrence of a whole range of groups of invertebrates,including useful pollinators and predators of cultivated plant pests. Last but not least, follows create a valuable habitat for different vertebrates, especially birds. Fallows are becoming then the islands of biodiversity in the agricultural landscape.
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The methodology of integrated Colorado potato beetle management in potatoes under new agro-environmental conditions
Doležal, P. ; Hausvater, E. ; Baštová, P. ; Kocourek, F. ; Stará, J. ; Sedlák, P. ; Sedláková, V. ; Skoková Habuštová, Oxana ; Nermuť, Jiří
The aim of the certified methodology is to provide updated principles of effective integrated Colorado potato beetle management for the agricultural practice resulting from new findings on biology and population genetics in the situation, when the range of effective insecticides has been reduced, pest resistance to insecticides has been increased and agro ecological conditions have been changed. Colorado potato beetle is a very serious insect pest of potatoes, highly mobile and able to quickly develop insecticide resistance. The methodology was drawn up based on the newest findings on the efficacy of existing insecticides registered against Colorado potato beetle, taking into account newly acquired knowledge in the field of pest resistance to active ingredients and a necessity of ever increasing multiple applications.
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Methodology for rapid, comprehensive, independent decision-making on the need, effectiveness and interaction of adaptation measures in river basins under climate change
Fischer, Milan ; Zeman, Evžen ; Vizina, A. ; Hanel, M. ; Bernsteinová, Jana ; Tachecí, P. ; Štěpánek, Petr ; Pavlík, P. ; Máca, P. ; Ghisi, Tomáš ; Rapantová, N. ; Bláhová, Monika ; Janál, P. ; Trnka, Miroslav
The aim of the methodology is to present methods for quantifying the impacts of projected climate change on the water balance when applying adaptation measures in the Czech Republic for the next few decades. Adaptation measures should contribute to the sustainability of the water balance in all major user segments of water use and management in the basin. The main principle is the use of hydrological models to transform climate change scenarios into time series of hydrological conditions and to quantify the overall water balance of the basin using different types of adaptation measures and their implementation over time. Special emphasis is placed on the evaluation of combinations of adaptation measures that cannot be analysed by simplified methods. The methodology is designed to search for the optimal combination of adaptation measures in the assessed catchment. The proposed approach eliminates the shortcomings of effectiveness assessment from the perspective of the exclusive user of the water resource, as the evaluation of the effectiveness of adaptation measures is carried out in the form of a multi-criteria analysis of the evaluation of the outputs of the simulation model for predicting the water balance in the whole basin. This methodology can be used to assess different adaptation measures in all basic segments of water users: agriculture, forestry, energy, water management and others.
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Methodology for determining the main disturbances in the water management balance and optimizing adaptation measures in the conditions climate change
Fischer, Milan ; Zeman, Evžen ; Vizina, A. ; Hanel, M. ; Bernsteinová, Jana ; Tachecí, P. ; Štěpánek, Petr ; Pavlík, P. ; Máca, P. ; Ghisi, Tomáš ; Rapantová, N. ; Bláhová, Monika ; Janál, P. ; Trnka, Miroslav
Ongoing climate change is causing a global increase in air temperature. While this is leading to an acceleration of the global hydrological cycle, and therefore a global increase in precipitation, the spatiotemporal variability in precipitation is much more complicated. While temperature in the Czech Republic shows a consistently increasing trend similar to that of surrounding countries and the planet as a whole, precipitation can be simplified that long-term averages of annual totals remain and are likely to remain very similar in the coming decades. Rising air temperatures inherently bring increased evaporative demand of the atmosphere and, for the same precipitation, a lower ratio of precipitation to evaporation, i.e. the climatic water balance shows a negative trend.
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