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Mikroskopické houby v půdě, listovém opadu, exkrementech a střevním traktu .i.Dendrobaena mrazeki./i. (Lumbricidae)
Nováková, Alena ; Pižl, Václav
Species diversity and quantitative parameters (mycelial length, CFU counts) of saprotrophic microfungi assemblages were studied in the intestine and casts of .i.D. mrazeki./i. and compared with those in soil and litter at four plots of a thermophilous oak forest in Southern Moravia (Czech Republic) in 2004. Soil dilution plate method and soil washing technique and three isolation media were used for the isolation of fungi. Differences in species number and composition of fungi assemblages were found both among individual substrates studied and between the results of different isolation methods. Number of species isolated by the soil dilution plate method was higher in earthworm casts than in soil, however, the opposite results were obtained using soil washing technique. CFU counts reached the highest values in leaf litter.
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Příspěvky k půdní zoologii ve střední Evropě I. Příspěvky ze 7. středoevropského půdně zoologického workshopu
Proceedings book involves contributions presented at the 7th Central European Workshop on Soil Zoology held in České Budějovice on April 14-16, 2003. Individual papers summarise actual results from partial disciplines of soil zoology and ecology, taxonomy and faunistic of individual groups of soil invertebrates. Several studies summarise information about the composition and changes of soil animal communities, both in natural and non disturbed grassland and forest soils in Central Europe as well as in soils differently influenced by human activities. A part of the papers deal with the distribution and ecology of cave invertebrates.
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Hlístice (Nematoda) v jílech hald uhelných dolů a v pokusně introdukovaných pásech luční půdy
Háněl, Ladislav
Soil nematodes were studied in coal-mining clay spoil denuded by a landslide of the upper part of a colliery dump three years ago. This control plot was compared with nematodes in introduced strips of fresh meadow soil heaped up on the spoil and in spoil strips between these soil strips. .i.Ecumenicus monohystera./i. (25% of all nematode individuals) and .i.Aporcelaimellus obtusicaudatus./i. (23%) dominated in the control spoil whereas in spoil between soil strips their population densities decreased. The greatest number of nematode species and genera was present in the soil strips. The number of species and genera increased in spoil strips adjacent to soil strips and proportions between trophic groups partially changed. Introduction of fresh meadow soil into colliery spoils may influence nematode assemblages in post-mining clays. Nevertheless, clay patches can harbour peculiar nematode assemblages that can contribute to the overall diversity of a post-mining landscape.
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Žížaly v ekotonu pole - les
Pižl, Václav ; Zeithaml, J.
The effects of a forest-field ecotone on the density, biomass and species composition of earthworm communities were assessed. Five sites (blocks) differing in type of crop rotation used in the field were studied near Kostelec nad Černými Lesy, Czech Republic. At each block, soil samples were taken in seven parallel rows perpendicular to a transect from the oak forest to the centre of the field. Individual rows were located: in the forest (5 m from the edge), on the foresf edge, and in the field in distances of 5,10, 25, 50, 100 m from the edge of the forest. Six samples were taken in each row in spring 2001. Digging and hand sorting of soil samples, followed by formalin application was used to extract earthworms. Nine species and subspecies of earthworms were identified, among which .i.Aporrectodea caliginosa./i. predominated. Per block, the species number varied from 5 to 7.
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Emissions of greenhouse gases (N2O and CO2) from pasture soil as a result of activity of soil microbial community - preliminary results
Šimek, Miloslav ; Hynšt, Jaroslav ; Brůček, Petr ; Čuhel, Jiří
Cattle overwintering area was shown an important point source of N2O and CO2. The emissions of N2O were mostly directly related to the rate of animal impact as the greatest fluxes of N2O and the highest total cumulative N2O emissions as well were found at the most impacted site. The fluxes of N2O were however very variable in time and most of N2O was emitted during several short events in spring or in winter. Laboratory experiments suggested that total amount of nitrogen escaping from soil from impacted sites is much greater than indicated by field measurements of N2O fluxes and molecular nitrogen (N2) was shown as the main nitrogen gas. It is hypothesized that soil pH is a crucial soil factor controlling the mole fraction of N2O: under slightly alkaline conditions in severely impacted soils, denitrification is completed and most of nitrogen escapes from the soil as N2; under more acidic conditions, however, the activity of nitrous oxide reductase is partly reduced.
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Dynamics of N2O emissions after C and N amendments of soil at the cattle overwintering area
Hynšt, Jaroslav ; Brůček, Petr ; Čuhel, Jiří ; Šimek, Miloslav
Field experiments were carried out at the cattle overwintering area in South Bohemia, Czech Republic, focused on the emissions of N2O after soil amendments with nitrate and glucose. Emissions were estimated using a permanent static chamber technique. Nitrate and glucose amendments caused very sharp increases of N2O fluxes, which however diminished after 2-3 days. Total amount of N2O escaping the soil was related to the amount of N and C added, and was calculated to 1-15 kg N.ha-1 (0.2-3% of N applied). Emissions were heterogeneous, which is shown by great differences among the individual chambers. Emissions were variable in time and very probably were controlled by temperature, as they approximately followed the air and surface soil temperature patterns. Despite the timing of amendment application, peaks of emissions were always determined 6 hours after the amendments.
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Stonožky smrkových lesů Moravskoslezských Beskyd, Česká republika
Wytwer, J. ; Tajovský, Karel
Centipede communities (Chilopoda) were studied in five spruce forests (.i.Picea abies./i.) in the Moravskoslezské Beskydy Mts., Northern Moravia, Czech Republic. Investigations were carried out from 1988 to 1993 and afterwards repeatedly in the years 1996-1997 in 5 to 134 year-old forest stands situated at the localities Bílý Kříž and Kněhyně. The stands differed in altitude, in air pollution and degree of tree defoliation. A total of 15 species of centipedes was found in all stands studied. The most abundant species occurring in all studied stands were .i.Lithobius mutabilis, L. forficatus, L. burzenlandicus burzenlandicus, Geophilus insculptus./i.. The centipedes .i.L. burzenlandicus burzenlandicus, G. insculptus./i. predominated mainly in the soil samples, .i.L. forficatus./i. was abundant in pitfall traps only. The mean densities of the whole centipede communities ranged from 9.0 to 194.6 ind.m-2 in 1988-1993, the highest densities were characteristic for the youngest spruce stand.
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Vývoj půdní fauny na loukách obnovených na orné půdě: Iniciální fáze sukcesního vývoje
Tajovský, Karel ; Pižl, Václav ; Starý, Josef ; Balík, Vladimír ; Frouz, Jan ; Schlaghamerský, J. ; Háněl, Ladislav ; Rusek, Josef ; Kalčík, Jiří
The development of soil fauna assemblages (testate amoebae, nematodes, enchytraeids, earthworms, terrestrial isopods, millipedes, centipedes, collembolans, dipteran larvae) has been investigated in a -field experiment since autumn 1999. Four types of treatment were studied in plots previously managed as arable land: 1) sowing with a native seed mixture, 2) sowing of a 5 m wide central strip with native and the lateral strips with commercial seed mixture, 3) sowing of a 5 m wide central strip with native seed mixture, leaving the lateral strips to spontaneous succession, and 4) leaving to spontaneous succession (abandoned field). The observed increase of qualitative and quantitative parameters of the individual soil animal groups corresponded to the initial phase of successional development. Eurytopic and euryvalent representatives as well as epigeic species of soil micro-, meso- and macrofauna prevailed in the studied treatments during the first years of observation.
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Soil macrofauna of mountain spruce stands in the Bohemian Forest as affected with selective tree cutting
Tajovský, Karel ; Pižl, Václav
Assemblages of soil macrofauna (earthworms, millipedes and centipedes) were studied in three specially protected mountain spruce stands in the Bohemian Forest (Czech Republic) in 2000-2003 using soil sampling to assess their quantitative and qualitative parameters. Spruce forests under study possessed poor (density 7,7-61,3 ind.m-2), but very specific earthworm assemblages built of 2-4 acidotolerant epigeic species. Similarly, assemblages of millipedes were low in both density (1,6-44,8 ind.m-2) and species numbers. Density of centipedes reached 41,1-206,4 ind.m-2, and their assemblages were composed mainly of the representatives of the order Geophilomorpha. Comparison of intact stands and those with selective cutting of spruce trees showed the negative impact of forestry management on populations of millipedes and centipedes. Density of both groups as well as species number of millipedes was markedly lower at selectively cut plot in the Trojmezná. On the contrary, denser earthworm populations were found at cut than at intact plots, most probably due to changes in herb vegetation and litter layer followed tree cutting.
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Soil microflora of spruce forests of the Bohemian Forest
Nováková, Alena ; Lukešová, Alena
Species composition and quantity of microalgae and micromycetes inhabiting soils of original spruce forest were studied in 3 localities of the Bohemian Forest (Trojmezná, Smrčina, Boubín) in years 2000-2003. Both intact plots, and those ones with selective tree cutting were compared in Trojmezná and Smrčina. In total, 46 species of algae and 72 taxa of micromycetes, represented by common soil species, were found in studied soils. Green algae (mainly Chlorophyceae and Charophyceae) prevailed both qualitatively and quantitatively among soil algae which was connected with low pH. All isolated micromycetes belonged to saprotrophic fungi. Total numbers of algal species ranged between 20-33 and total algal abundance between 48,1-4210,0 cells per g of dry soil in studied plots. Higher algal species richness was recorded in plots with selective tree cutting then in intact plots. Oposite trend of average total algal abundance was observed, despite big variations in particular sampling occasions. Numbers of taxa of saprophytic mycromycetes ranged between 32-40, lenght of mycelium between 6,1-84,5 m per g of dry soil, and numbers of CFU between 57,5-2413,4. No differences in species composition and quantity of saprotrophic micromycetes between intact plots and plots with tree cutting were found.
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