Original title: Expression, purification and crystallization of the putative transcriptional regulator MSMEG_6227 from Mycobacteria
Authors: HALAS, Simon
Document type: Bachelor's theses
Year: 2021
Language: eng
Abstract: MSMEG_6227, a putative transcriptional regulator from Mycobacterium smegmatis, was cloned and expressed in E. coli BL21 cells. The expressed protein was purified by chromatographic methods and an optimized protocol for the expression and purification of MSMEG_6227 was established. With the purified protein initial crystallization trials were set up, which need to be further optimized and scaled up in the future to resolve the X-ray structure of MSMEG_6227.
Keywords: MSMEG_6227; Mycobacteria; Mycobacterium smegmatis; putative transcriptional regulator
Citation: HALAS, Simon. Expression, purification and crystallization of the putative transcriptional regulator MSMEG_6227 from Mycobacteria. České Budějovice, 2021. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/59302

Permalink: http://www.nusl.cz/ntk/nusl-515486


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses