Název:
An appropriate method for assessing hydrogel pore sizes by cryo-sem
Autoři:
Adámková, Kateřina ; Trudicová, M. ; Hrubanová, Kamila ; Sedláček, P. ; Krzyžánek, Vladislav Typ dokumentu: Příspěvky z konference Konference/Akce: Anniversary International Conference on Nanomaterials - Research and Application (NANOCON) /10./, Brno (CZ), 20181017
Rok:
2019
Jazyk:
eng
Abstrakt: The aim of our work was to examine and describe ultrastructure of the agarose hydrogel and any possible structural concentration dependencies, and to assess the distribution and size of pores of agarose hydrogel in dependence on its concentration. Four concentrations were prepared (0.5 %, 1.0 %, 2.0 % and 4.0 % of dry weight content) and cryo-SEM and turbidimetry methods were executed on wet (original) samples in order to image the ultrastructure and measure the pore sizes within. \nReasonable results were obtained for the wet samples as they were closer to their native state they are usually used for applications in. Cryo-SEM and turbidimetry provided comparable results of pore diameters and allowed to compare pore diameters dependant on the concentrations, moreover, it showed more detailed and realistic structure.
Klíčová slova:
agarose; cryo-SEM; freezing methods; hydrogelcryo-SEM; image analysis Číslo projektu: GA17-15451S (CEP), LO1212 (CEP), ED0017/01/01 Poskytovatel projektu: GA ČR, GA MŠk, GA MŠk Zdrojový dokument: 10th Anniversary International Conference on Nanomaterials - Research and Application (NANOCON 2018), ISBN 978-80-87294-89-5
Instituce: Ústav přístrojové techniky AV ČR
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Informace o dostupnosti dokumentu:
Dokument je dostupný v příslušném ústavu Akademie věd ČR. Původní záznam: http://hdl.handle.net/11104/0309123