Epigenetická regulace adhezivních molekul u high-grade serózního ovariálního karcinomu

Baranová, Ivana

5 Summary Title: Epigenetic Regulation of Adhesive Molecules in High-grade Serous Ovarian Carcinoma The lack of effective biomarkers for screening and early detection of ovarian cancer is currently considered as one of the most pressing problems in oncogynecology. Because epigenetic alterations occur early in the cancer development, they provide great potential to serve as such biomarkers. Epigenetic mechanisms have been implicated also in regulation of adhesion molecules that play a major role in cancer progression. The main aim of this study was to investigate the methylation pattern of selected cadherin and catenin genes in ovarian cancer tissue by comparison with control tissue. The study group consisted of 68 patients with high-grade serous ovarian cancer (HGSOC) and 46 control patients. To determine the sites with the most significant methylation in selected genes next-generation sequencing was employed. For further confirmation of detected methylation of selected regions, methylation-sensitive high-resolution melting analysis and real-time methylation-specific polymerase chain reaction were used. In attempt to design potential biomarker panel for future screening of HGSOC as the secondary aim of our study, cadherins were evaluated together with transcription factors from our previous study....

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