Original title: Image Analysis of Gene Locus Positions Within Chromosome Territories in Human Lymphocytes
Authors: Stepka, K. ; Falk, Martin
Document type: Papers
Conference/Event: 9th International Doctoral Workshop on Mathematical and Engineering Methods in Computer Science (MEMICS), Telč (CZ), 20141017
Year: 2014
Language: eng
Series: Lecture Notes in Computer Science
Abstract: One of the important areas of current cellular research with substantial impacts on medicine is analyzing the spatial organization of genetic material within the cell nuclei. Higher-order chromatin structure has been shown to play essential roles in regulating fundamental cellular processes, like DNA transcription, replication, and repair. In this paper, we present an image analysis method for the localization of gene loci with regard to chromosomal territories they occupy in 3D confocal microscopy images. We show that the segmentation of the territories to obtain a precise position of the gene relative to a hard territory boundary may lead to undesirable bias in the results, instead, we propose an approach based on the evaluation of the relative chromatin density at the site of the gene loci. This method yields softer, fuzzier ´boundaries´, characterized by progressively decreasing chromatin density. The method therefore focuses on the extent to which the signals are located inside the territories, rather than a hard yes/no classification.
Keywords: deletion; selection method
Host item entry: MATHEMATICAL AND ENGINEERING METHODS IN COMPUTER SCIENCE, MEMICS 2014, ISBN 978-3-319-14895-3, ISSN 0302-9743

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297912

Permalink: http://www.nusl.cz/ntk/nusl-399148


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Research > Institutes ASCR > Institute of Biophysics
Conference materials > Papers