Original title: ANALYSIS OF SEQUENCE SPECIFIC INTERACTIONS BETWEEN DNA AND P53 FAMILY PROTEINS BY ELISA, SLOT-BLOT AND EMSA
Authors: Adámik, Matěj ; Holanová, L. ; Navrátilová, Lucie ; Nygrinova, J. ; Pokorova, J. ; Petr, Marek ; Tichý, Vlastimil ; Brázdová, Marie
Document type: Papers
Conference/Event: CECE 2014. International Interdisciplinary Meeting on Bioanalysis /11./, Brno (CZ), 2014-10-20 / 2014-10-22
Year: 2014
Language: eng
Abstract: DNA-protein interactions of core domains of p53, p63 and p73, members of tumor suppressor p53 family, were investigated by multiple methods with regard to verifying the sequence specificity with which short target oligonucleotides/long DNA fragments can be recognized. The sequence specificity of core domains and wtp53 full protein binding to specific sequence in both types of DNA substrates was confirmed in solution, on surface and in gels by ELISA, slot-blot and EMSA.
Keywords: binding; p63
Host item entry: CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-2-0

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297915

Permalink: http://www.nusl.cz/ntk/nusl-396433


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Research > Institutes ASCR > Institute of Biophysics
Conference materials > Papers
 Record created 2019-07-25, last modified 2021-11-24


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