Original title: Possibility of Using Purine Oxidation Signals for DNA Sequence Analysis
Authors: Špaček, Jan ; Cahová, Kateřina ; Havran, Luděk ; Fojta, Miroslav
Document type: Papers
Conference/Event: Moderní elektrochemické metody /35./, Jetřichovice (CZ), 20150518
Year: 2015
Language: eng
Abstract: Signals provided by electrochemical oxidation of single stranding DNA on the surface of pyrolytic graphite electrode, which correspond to the oxidation of adenine and guanine (A and G) are known for over forty years. The mechanism of oxidation of free A and G as well as nucleoside mono-, and triphosphates of these bases is know but the mechanism of oxidation of purines in the DNA still remains unexplained. Ratiometry of A and G signals could be a new tool for DNA sequence analysis, if signals of A and G oxidation obtained from single-stranded DNA adsorbed at the electrode surface are proportional to the number of oxidized As and Gs in studied samples. Our preliminary results suggest that there is a correlation, yet we encountered problems, which yet has to be addressed and explained before this method could be applied for DNA analysis.
Keywords: DNA oxidation; DNA sequence analysis; Electrochemistry
Project no.: GBP206/12/G151 (CEP)
Funding provider: GA ČR
Host item entry: XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference, ISBN 978-80-905221-3-8

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297905

Permalink: http://www.nusl.cz/ntk/nusl-396426


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