Original title: Studies of Protein-DNA Interactions using Immunoprecipitation with DNA Probes Labelled with Electroactive Groups
Authors: Hermanová, Monika ; Špaček, Jan ; Pivoňková, Hana ; Fojta, Miroslav
Document type: Papers
Conference/Event: Moderní elektrochemické metody /35./, Jetřichovice (CZ), 20150518
Year: 2015
Language: eng
Abstract: Different electrochemically active species were tested for labelling of DNA probes and detection of DNA-protein binding. As the DNA probes, oligonucleotides bearing or lacking specific binding site of the p53 protein were chosen. They were tail-labelled either via chemical modification of single-stranded (ss) oligo(dT) DNA tails with an oxoosmium complex, or via enzymatic synthesis of the ss tail by terminal deoxynucleotidyl transferase and deoxynucleoside triphosphates modified with electrochemically reducible moieties. Electrochemical detection enabled to discriminate between sequence-specific and non-specific p53-DNA binding.
Keywords: electrochemical detection; p53; Protein-DNA binding
Project no.: GBP206/12/G151 (CEP)
Funding provider: GA ČR
Host item entry: XXXV. Moderní elektrochemické metody. Sborník přednášek mezinárodní odborné konference, ISBN 978-80-905221-3-8

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297902

Permalink: http://www.nusl.cz/ntk/nusl-396422


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Research > Institutes ASCR > Institute of Biophysics
Conference materials > Papers