Original title: Constant Current Chronopotentiometry as a Method for Detection of Singlet Oxygen Protein Damage
Authors: Ostatná, Veronika ; Vargová, Veronika ; Černocká, Hana ; Paleček, Emil
Document type: Papers
Conference/Event: Moderní elektrochemické metody /36./, Jetřichovice (CZ), 20160523
Year: 2016
Language: eng
Abstract: Proteins are one of the major targets for oxidative damage in the cell.Indirect non-radical oxidation of the protein via formation and subsequent reaction with single oxygen (O-1(2))is one of the major processes.In this work we studied the single oxygen(O-1(2))-mediated oxidation of bovine serum albumin (BSA) by constant current chronopotentiometry in combination with mercury electrode.Our chronopotentiometric data show that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Our method utilizing intrinsic electrochemical signal of proteins provides a sensitive detection methods for minor damages in various proteins.
Keywords: affinity tags; carbon electrodes; mercury; purification
Project no.: GA13-00956S (CEP)
Funding provider: GA ČR
Host item entry: XXXVI. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-4-5

Institution: Institute of Biophysics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0297882

Permalink: http://www.nusl.cz/ntk/nusl-396406


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Research > Institutes ASCR > Institute of Biophysics
Conference materials > Papers
 Record created 2019-07-25, last modified 2022-09-29


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