Original title:
Constant Current Chronopotentiometry as a Method for Detection of Singlet Oxygen Protein Damage
Authors:
Ostatná, Veronika ; Vargová, Veronika ; Černocká, Hana ; Paleček, Emil Document type: Papers Conference/Event: Moderní elektrochemické metody /36./, Jetřichovice (CZ), 20160523
Year:
2016
Language:
eng Abstract:
Proteins are one of the major targets for oxidative damage in the cell.Indirect non-radical oxidation of the protein via formation and subsequent reaction with single oxygen (O-1(2))is one of the major processes.In this work we studied the single oxygen(O-1(2))-mediated oxidation of bovine serum albumin (BSA) by constant current chronopotentiometry in combination with mercury electrode.Our chronopotentiometric data show that photo-oxidized BSA was more susceptible to the electric field-induced denaturation than non-oxidized native BSA. Our method utilizing intrinsic electrochemical signal of proteins provides a sensitive detection methods for minor damages in various proteins.
Keywords:
affinity tags; carbon electrodes; mercury; purification Project no.: GA13-00956S (CEP) Funding provider: GA ČR Host item entry: XXXVI. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-4-5
Institution: Institute of Biophysics AS ČR
(web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences. Original record: http://hdl.handle.net/11104/0297882