Original title: Construction and Application of Flow Amperometric Biosensor Based on Enzymatic Reactor for Determination of Choline
Authors: Tvorynska, Sofiia ; Barek, J. ; Josypčuk, Bohdan
Document type: Papers
Conference/Event: Moderní elektrochemické metody /39./, Jetřichovice (CZ), 20190520
Year: 2019
Language: eng
Abstract: In this contribution, a novel fast, simple and stable biosensor with the enzymatic reactor based on choline oxidase (ChOx) was developed and applied for the determination of choline using flow injection analysis with amperometric detection. The preparation of the reactor is based on the covalent immobilization of ChOx with glutaraldehyde to mesoporous silica powder (SBA-15) previously covered by NH2-groups. The experimental parameters affecting the sensitivity and stability of the biosensor were optimized. The proposed biosensor with the newly developed ChOx-based reactor possesses good repeatability, reproducibility, long-term stability, and reusability. It was successfully applied for the determination of choline in commercial pharmaceuticals.
Keywords: choline; choline oxidase; enzymatic reactor; Flow analysis
Project no.: GA17-05387S (CEP)
Funding provider: GA ČR
Host item entry: XXXIX. Moderní elektrochemické metody. Sborník přednášek, ISBN 978-80-905221-7-6

Institution: J. Heyrovsky Institute of Physical Chemistry AS ČR (web)
Document availability information: Fulltext is available on demand via the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0296751

Permalink: http://www.nusl.cz/ntk/nusl-395989


The record appears in these collections:
Research > Institutes ASCR > J. Heyrovsky Institute of Physical Chemistry
Conference materials > Papers