Original title: Mechanisms of dsRNA virus replication: Cloning, production and structural characterization of C-terminal domain of sigmaNS.
Authors: MANOCZKI, Nelli Vanessza
Document type: Bachelor's theses
Year: 2019
Language: eng
Abstract: A gene fragment encoding the C-terminal domain of the sigmaNS protein of the avian reovirus was amplified via PCR. The amplicon was cloned using BsaI restriction enzyme into the pASKIBA37+ expression vector and transformed into One ShotTM TOP 10 Chemically Competent E.coli cells. Colony PCR was performed and plasmids were sent for sequencing. Sequence verified plasmids were transformed into One ShotTM BL21 (DE3) or Rosetta-gami B (DE3) Chemically Competent E.coli cells. During Pilot expressions the conditions for production of soluble recombinant protein were optimized and according to them the recombinant protein was produced in large scale. The presence of the sigmaNS-terminal domain was verfied by Western Blot.
Citation: MANOCZKI, Nelli Vanessza. Mechanisms of dsRNA virus replication: Cloning, production and structural characterization of C-terminal domain of sigmaNS.. České Budějovice, 2019. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/52812

Permalink: http://www.nusl.cz/ntk/nusl-395125


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses