Original title: A Microfluidic devide for monitoring cell migration
Authors: Ledvina, Vojtěch ; Klepárník, Karel ; Legartová, Soňa ; Bártová, Eva
Document type: Papers
Conference/Event: CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./, Brno (CZ), 20181015
Year: 2018
Language: eng
Abstract: A polydimethylsiloxane microfluidic device for monitoring directed cell migration was developed. The device comprises a main channel barred by pillar arrays with decreasing spacing and utilizes chemotactic movement of cells in the direction of increasing gradient of fetal bovine serum. HeLa cell line transfected with Premo FUCCI Cell Cycle Sensor was used as a model organism. The cells were imaged for several days using a scanning confocal microscope and the rate of migration in different cell cycle phases was evaluated.
Keywords: cell migration; microfluidic device; Premo FUCCI Cell Cycle Sensor
Project no.: GA17-01995S (CEP)
Funding provider: GA ČR
Host item entry: CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-5-1

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available at external website.
External URL: http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf
Original record: http://hdl.handle.net/11104/0290940

Permalink: http://www.nusl.cz/ntk/nusl-390739


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers