Original title:
Microfluidic device for cell counting and electrical lysis
Authors:
Václavek, Tomáš ; Křenková, Jana ; Foret, František Document type: Papers Conference/Event: CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./, Brno (CZ), 20181015
Year:
2018
Language:
eng Abstract:
This lecture presents two microfluidic devices as essential units for single cell proteomic analysis, firstly a device for cell counting based on resistive pulse sensing as an alternative to optical platforms based on visual control over the sample input, secondly a unit for electrical lysis of cells using direct current voltage pulses to lyse cells one by one with a possibility to analyze the distinct lysate zones by electrospray ionization mass spectrometry.
Keywords:
cell lysis; electrospray ionization mass spectrometry; microextraction of basic drugs; microfluidic devices; single cell proteomic analysis Project no.: GJ16-09283Y (CEP), GBP206/12/G014 (CEP) Funding provider: GA ČR, GA ČR Host item entry: CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-5-1