Studium inhibičního (toxického) vlivu alkaloidů vybraných druhů rostlin z čeledi Amaryllidaceae na některé lidské enzymové systémy (in vitro studie) II.

Vaněčková, Nina

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany Candidate: Mgr. Nina Vaněčková Supervisor: Doc. Ing. Lucie Cahlíková, PhD. Title of Doctoral Thesis: Study of the inhibitory (toxic) effect of the alkaloids from chosen plants of Amaryllidaceae family on some human enzymatic systems (in vitro study) II. Key words: Nerine bowdenii, Amaryllidaceae, alkaloids, biological activity, acetylcholinesterase, butyrylcholinesterase, prolyl oligopeptidase, cytotoxicity An alkaloid extract prepared from the fresh bulbs of Nerine bowdenii Watson was chosen as a source of a variety of Amaryllidaceae alkaloids with a potential for interesting biological activity. The mixture of alkaloids, prepared by standard extraction techniques, was fractionated by column chromatography on aluminium oxide using step gradient elution with petrol, chloroform and ethanol. The isolation of 21 alkaloids was a result of column chromatography, preparative TLC and crystallizations. Two of these alkaloids were isolated from this plant for the first time. The chemical structures of isolated compounds were determined on the basis of spectrometric techniques (NMR, MS, optical rotation) and by comparison with literature data. If the amount of the isolated alkaloid were sufficient, it was...

host :: přihlásit Digitální repozitář
		Hledej		Nový záznam		Nápověda		O repozitáři