Original title: Porovnání specifické exprese u biofilmů bakterií a kvasinek
Translated title: Comparison of specific expression in bacterial and yeast biofilms
Authors: Kicko, Peter ; Palková, Zdena (advisor) ; Lichá, Irena (referee)
Document type: Bachelor's theses
Year: 2018
Language: slo
Abstract: [eng] [cze]
The development and maintenance of biofilm is a complex process that is based on a change in genetic expression. The biofilm formation is observed in some prokaryotic and eukaryotic cells. During its formation, cell aggregation occurs, extracellular matrix is created and we observe the formation of metabolically differentiable cells, often with increased resistance to antimicrobial drugs. This work focuses on important steps leading to biofilm formation associated with specific gene expression and highlights the similar and different processes between bacterial and yeast cells. The work begins by comparison of cell signalling, it continues by comparing the expression of the adhesive proteins and extracellular enzymes, synthesis of exopolysaccharides, formation of extracellular nucleic acid, and in the last chapter we focused on the formation of persistors. The aim of this work is to connect the acquired information and to contribute to the understanding the complexity of this process. Key words: biofilm, signalling, adhesins, exopolysaccharides, extracellular nucleic acid, persistor Tvorba a udržanie biofilmu je komplexný proces, ktorý je podmienený zmenou genetickej expresie. Formovanie biofilmu je pozorované u niektorých prokaryotických i eukaryotických buniek. V priebehu jeho tvorby dochádza k zoskupovaniu buniek, vzniku extracelulárnej matrix a vytvoreniu metabolicky odlíšiteľných buniek častokrát so zvýšenou rezistenciou na antimikrobiálne látky. Táto práca sa zameriava na významné kroky vedúce k vytvoreniu biofilmu spojené so špecifickou genetickou expresiou a poukazuje na podobné a rozdielne procesy u bakteriálnych a kvasinkových buniek. Práca začína porovnaním bunkovej signalizácie, pokračuje porovnaním expresie adhéznych proteínov a extracelulárnych enzýmov, syntézou exopolysacharidov, vznikom extracelulárnej nukleovej kyseliny a v poslednej kapitole je priblížené formovanie perzistorov. Cieľom tejto práce je spojiť získané informácie a prispieť k pochopeniu komplexnosti tohto procesu. Kľúčové slová: biofilm, signalizácia, adhezíny, exopolysacharidy, extracelulárna nukleová kyselina, perzistor
Keywords: adhesins; biofilm; exopolysaccharides; extracellular nucleic acid; persistor; signalling; adhezíny; biofilm; exopolysacharidy; extracelulárna nukleová kyselina; perzistor; signalizácia

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/101448

Permalink: http://www.nusl.cz/ntk/nusl-387382


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Bachelor's theses