Original title:
Alergenogenní epitopy u klíšťat
Authors:
PEJŠOVÁ, Hana Document type: Bachelor's theses
Year:
2018
Language:
cze Abstract:
In this study we focused on detection of galactose-alpha-1,3-galactose (alpha-gal epitope) as to determine the proteins which carry this glycan in ticks. Affinity chromatography with magnetic beads was used to purify the proteins from tick cell lines. The proteins obtained were visualized by SDS-PAGE and western blotting. Purified proteins were then identified using mass spectometry. Fluorescence microscopy was also used to detect Gal as well as another allergenogenic epitope, the core alpha-1,3-fucose, in ticks cells. Citation: PEJŠOVÁ, Hana. Alergenogenní epitopy u klíšťat. České Budějovice, 2018. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice
(web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia. Original record: http://www.jcu.cz/vskp/52438