Original title: New Microbial Glycosidases
Translated title: New Microbial Glycosidases
Authors: Charvátová, Andrea ; Křen, Vladimír (advisor) ; Králová, Blanka (referee) ; Walterová, Daniela (referee)
Document type: Doctoral theses
Year: 2007
Language: eng
Abstract: Sunrvranv Glycosidases from fungi are useful in the preparation of various glycosides mainly by transglycosylation or reversed glycosylation. The lack ofany requirement for protection- deprotection sequences, mild conditions and easier synthesis of thermodynamically not preferred glycosidic bonds are the main advantages of glycosidase-catalysed synthesis of glycosides. In ttris thesis we concentratedon exoglycosidases,mainly B.N.aceýlhexosaminidases, cr-galactosidases and cr-L-rhamnosidases. For the enzyme preparation taxonomically characterised fungal strains from public collections were used. A library comprising more than 200 various glycosidases was developed by modification ofcultivation conditions and by the use ofspecific inducers. The enrymes were used for screening ofsubstrate specificity and stability in organic solvents and subsequently for synthesis and modification of various substrates. Both B-N-acetylgalactosaminidase and B-N-acetylglucosaminidase activities in the series of B-N-acetylhexosaminidases were determined. Saccharides with strong immunomodulation activity $-o-GalpNAc-(i-+4)-o-GlcpNAc and B-o-GalpNAc-(1-+6)-o-GlcpNAc were synthesisedby transglycosylation using B-N-acetylhexosaminidase from Penicillium oxalicum CCF 2430' enryme having the highest B.N-acetylgalactosaminidase activiý....

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/95151

Permalink: http://www.nusl.cz/ntk/nusl-373560


The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Doctoral theses