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Original title:
In vivo study of diatom assemblages using low temperature method for ESEM
Authors: Tihlaříková, Eva ; Neděla, Vilém ; Fránková, Markéta
Document type: Papers
Conference/Event: Multinational Congress on Microscopy /13./, Rovinj (HR), 20170924
Year: 2017
Language: eng
Abstract: Diatoms are the most species-rich algal group represented by 12 000 described species and are recognized as powerful bio indicators and used for water quality monitoring. Diatom taxonomy is predominantly based on the morphology of ornate silicified cell wall called frustule composed of two overlapping parts (thecae). This frustule displays intricate patterns and designs unique to each species. For these studies conventional scanning electron microscopy (SEM) was and still is widely used. This method requires cleaning of diatom frustules in strong acids and peroxides followed by conductive coating.This aggressive procedure removes protoplast and damages delicate structures. Environmental scanning electron microscopy (ESEM) brings advantages of observation of fresh diatom material that are presence of whole intact diatom cells, not only empty diatom frustules, extracellular mucilaginous diatom secrets (e.g. pads, stalks, tubes) and whole diatom assemblages directly in situ together with other algal assemblages (e.g. cyanobacteria or green algae). However, in diatom research, ESEM is used mainly for elimination of conductive coating and observation of these organisms in their native wet state adhered directly on the host plant is not yet common. The ESEM observation of native aquatic samples is usually affected with radiation damage that can be lovered by the use of special methods like the Low Temperature Method (LTM) for the ESEM.
Keywords: diatom; ESEM; LTM; morphology
Project no.: LO1212 (CEP), ED0017/01/01
Funding provider: GA MŠk, GA MŠk
Host item entry: 13th Multinational Congress on Microscopy: Book of Abstracts, ISBN 978-953-7941-19-2
Institution: Institute of Scientific Instruments AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0277119
Permalink: http://www.nusl.cz/ntk/nusl-373316
The record appears in these collections:
Research > Institutes ASCR > Institute of Scientific Instruments
Conference materials > Papers
Authors: Tihlaříková, Eva ; Neděla, Vilém ; Fránková, Markéta
Document type: Papers
Conference/Event: Multinational Congress on Microscopy /13./, Rovinj (HR), 20170924
Year: 2017
Language: eng
Abstract: Diatoms are the most species-rich algal group represented by 12 000 described species and are recognized as powerful bio indicators and used for water quality monitoring. Diatom taxonomy is predominantly based on the morphology of ornate silicified cell wall called frustule composed of two overlapping parts (thecae). This frustule displays intricate patterns and designs unique to each species. For these studies conventional scanning electron microscopy (SEM) was and still is widely used. This method requires cleaning of diatom frustules in strong acids and peroxides followed by conductive coating.This aggressive procedure removes protoplast and damages delicate structures. Environmental scanning electron microscopy (ESEM) brings advantages of observation of fresh diatom material that are presence of whole intact diatom cells, not only empty diatom frustules, extracellular mucilaginous diatom secrets (e.g. pads, stalks, tubes) and whole diatom assemblages directly in situ together with other algal assemblages (e.g. cyanobacteria or green algae). However, in diatom research, ESEM is used mainly for elimination of conductive coating and observation of these organisms in their native wet state adhered directly on the host plant is not yet common. The ESEM observation of native aquatic samples is usually affected with radiation damage that can be lovered by the use of special methods like the Low Temperature Method (LTM) for the ESEM.
Keywords: diatom; ESEM; LTM; morphology
Project no.: LO1212 (CEP), ED0017/01/01
Funding provider: GA MŠk, GA MŠk
Host item entry: 13th Multinational Congress on Microscopy: Book of Abstracts, ISBN 978-953-7941-19-2
Institution: Institute of Scientific Instruments AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0277119
Permalink: http://www.nusl.cz/ntk/nusl-373316
The record appears in these collections:
Research > Institutes ASCR > Institute of Scientific Instruments
Conference materials > Papers
Record created 2018-03-09, last modified 2021-11-24