Original title: Activation of translational system during cell differentiation of streptomycetes.
Translated title: Aktivace translačního systému v průběhu buněčné diferenciace streptomycet
Authors: Bobek, Jan ; Palečková, Petra ; Vohradský, Jiří ; Mikulík, Karel
Document type: Papers
Conference/Event: International Symposium on the Genetics of Industrial Microorganisms /10./, Praha (CZ), 2006-06-24 / 2006-06-28
Year: 2006
Language: eng
Abstract: [eng] [cze]
Synchronously germinating spores of S. granaticolor were used to study the activation of translational system and expression of proteins. Proteomic approach was used to gain more information on expression of proteins during germination on two types of media differing in ability of cells to produce antibiotic granaticin. The requirement of functional trans-translational system for reactivation of translation in S. aureofaciens exposed to unfavourable conditions was examined. Abundance of tmRNA is connected with changes in the rate of translation and with the accumulation of tetracycline. Our data indicate that trans-translation could be involved in self-defence against antibiotics synthesized Synchronně germinující spory S. granaticolor byly použity ke studiu aktivace translačního systému a exprese proteinů. Proteomickou analýzou byla studována exprese proteinů na dvou typech media lišících se ve schopnosti buněk produkovat antibiotikum granaticin. Byla studována potřeba funkčního trans-translačního systému na reaktivaci translace u S. aureofaciens vystaveným nepříznivým podmínkám. Hladina tmRNA je spojena se změnami v rychlosti translace a produkcí tetracyklinu. Výsledky naznačují, že trans-translační systém je zahrnut v mechanismech resistence proti syntetizovaným antibiotikům
Keywords: streptomycetes; translation
Project no.: CEZ:AV0Z50200510 (CEP), GA203/05/0106 (CEP)
Funding provider: GA ČR
Host item entry: International Symposium on the Genetics of Industrial Microorganisms /10./

Institution: Institute of Microbiology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0146110

Permalink: http://www.nusl.cz/ntk/nusl-37132


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Research > Institutes ASCR > Institute of Microbiology
Conference materials > Papers