Original title: L01 DNA damage formation and DNA repair following an intervention of colorectal cell lines with ganoderma lucidum
Authors: Vodička, Pavel ; Opattová, Alena ; Čumová, Andrea ; Slíva, D.
Document type: Papers
Conference/Event: 16th International Nutrition and Diagnostics conference., Praha (CZ), 20161003
Year: 2016
Language: eng
Abstract: Colorectal cancer (CRC) is the third most common malignancy in the world and second most common cause of cancer related deaths in Europe. CRC is complex disease that develops as consequence of environmental and health risk factors with involvement of suboptimal DNA repair, resulting in an accumulation of DNA damage. Reactive oxygen species (ROS) are highly reactive molecules strictly controlled by cellular antioxidant system. Disturbance in the prooxidation–antioxidation homeostasis increases an extent of ROS and consequently an accumulation of DNA damage as well as apoptosis. \nMany natural compounds possess anti-cancer activities tentatively mediated by the generation of ROS. Cancer cells are more sensitive to oxidative DNA damage than non-malignant ones. Modulation of oxidative DNA damage and its repair by natural compounds may lead to selective cancer cell-death and further sensitization of cancer cells to the treatment. Ganoderma Lucidum (GLC) (Reishi, Ling-Zhi), a mushroom used in Chinese medicine for thousands of years, represents an example of a natural compound with empirically recorded anti-cancer as well as anti-proliferative effects. \nThe aim of our study is to define effect of Ganoderma lucidum (GLC) extract on DNA damage and DNA repair system in colorectal cell lines with different genetic backgrounds.\nOur results suggest that GLC extract decreases activity of the cellular antioxidant system which leads to oxidative DNA damage. GLC extract increases genotoxic burden in colorectal cancer cell lines, highlighted by the suppressed base excision repair capacity. These data indicate that specific oxidative DNA damage caused by natural compounds may become a potential tool for the improvement of specific anti-cancer treatment.\n
Keywords: 5-fluorouracil; colorectal cancer; colorectal cell lines; DNA repair; natural compound; oxidative DNA damage
Project no.: LH13061 (CEP), GA15-14789S (CEP), NV15-27580A (CEP), LD14050 (CEP)
Funding provider: GA MŠk, GA ČR, GA MZd, GA MŠk
Host item entry: International Nutrition and Diagnostics conference /16./, ISBN 978-80-7560-016-5

Institution: Institute of Experimental Medicine AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0273398

Permalink: http://www.nusl.cz/ntk/nusl-361675


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