Original title: Molekulární modelování lipidových membrán s fluorescenčními sondami
Translated title: Molecular modeling of lipid membranes with fluorescent probes
Authors: Dékány Fraňová, Miroslava ; Pospíšil, Miroslav (advisor) ; Vácha, Robert (referee) ; Barvík, Ivan (referee)
Document type: Doctoral theses
Year: 2014
Language: eng
Abstract: [eng] [cze]
Title: Molecular modeling of lipid membranes with fluorescent probes Author: RNDr. Miroslava Dékány Fraňová Department / Institute: Department of Chemical Physics and Optics MFF UK Supervisor of the doctoral thesis: RNDr. Miroslav Pospíšil, Ph.D., Department of Chemical Physics and Optics, MFF UK Abstract: We studied biological membranes with fluorescent probes. First part of the work describes the properties of lipid bilayer consisting of DPPC (dipalmitoylphosphatidylcholine) and various cholesterol concentrations (5 mol % and 20 mol %). The properties are studied via the free probe - DPH (diphenylhexatriene) which is in various concentrations randomly immersed into both layers of the membrane. Second part of this work studies the properties of DOPC (dioleoylphosphatidylcholine) membrane via pyrene probes attached to 4th , 6th , 8th , and 10th carbon atom in both acyl chains of the host lipid and compares how the membrane properties differ based on the various pyrene positions. Here we focused also on dimerization rate of pyrene probes based on their position and the relationship with lateral pressure profile. Keywords: molecular simulation, membrane, fluorescent probes, lipids, lateral pressure profile Název práce: Molekulární modelování lipidových membrán s fluorescenčními sondami Autor: RNDr. Miroslava Dékány Fraňová Katedra / Ústav: Katedra chemické fyziky a optiky MFF UK Vedoucí doktorské práce: RNDr. Miroslav Pospíšil, Ph.D., Katedra chemické fyziky a optiky MFF UK Abstrakt: Skúmali sme biologické membrány pomocou fluorescenčných sond. Prvá časť práce opisuje vlastnosti lipidovej dvojvrstvy zloženej z molekúl DPPC (dipalmitoylfosfatidylcholín) a rôznej koncentrácie cholesterolu (5 mol % a 20 mol %) skúmaných pomocou sondy DPH (difenylhexatrién), ktorá je v rôznych koncentráciách ponorená do oboch vrstiev membrány. Druhá časť práce skúma vlastnosti DOPC (dioleoylfosfatidylcholín) membrány pomocou pyrénovej sondy umiestnenej na 4., 6., 8. a 10. uhlík oboch acylových reťazcov hosťujúceho lipidu a porovnáva ako sa vlastnosti membrány menia v závislosti od pozície pyrénu. Zamerali sme sa aj na dimerizáciu pyrénov v závislosti od ich pozície a zisťovali vzťah s profilom laterálneho tlaku. Klíčová slova: molekulární simulace, membrána, fluorescenční sonda, lipidy, profil laterálneho tlaku

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/72968

Permalink: http://www.nusl.cz/ntk/nusl-342328


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Doctoral theses