Original title: Využití tuhých elektrod na bázi uhlíku k elektrochemické oxidaci acikloviru
Translated title: Utilization of carbon-based solid electrodes to electrochemical oxidation of acyclovir
Authors: Vaněčková, Eva ; Schwarzová, Karolina (advisor) ; Fischer, Jan (referee)
Document type: Master’s theses
Year: 2014
Language: cze
Abstract: Electrochemical oxidation of acyclovir at glassy-carbone electrode and boron-doped diamond electrode gives one pH-dependent oxidation signal; the corresponding electrode reaction is controlled by diffusion. It is followed by a second indistinctive signal in the pH range 2,0 - 9,0. Further, optimization of conditions was carried out for determination of acyclovir using DC and DP voltammetry. The calibration dependence on GC electrode in B−R buffer (pH = 6,0) is linear only in the concentration range from 2 to 10 μmol∙l−1 for DCV with limit of detection 0,38 μmol∙l−1; for DPV it is not linear. BDD electrode has to be activated to prevent its passivation. Anodic activation at +2,4 V for 15 s with simultaneous stirring of solution results in relative standard deviation of 0,9% for acyclovir concentration of 1∙10−4 mol∙l−1. Using DCV and DPV methods linear concentration dependences were obtained in 0,1 mol∙l-1 nitric acid and in pH 6,0 B−R buffer. The lowest limit of detection and limit of quantification (LOD = 0,47 μmol∙l−1 a LOQ = 1,55 μmol∙l−1) and linear range from 0,6 to 100 μmol∙l−1 was reached in pH 6,0 B−R buffer. Further, standard addition method was used to quantify acyclovir in Zovirax tablets. Satisfactory recoveries of 101,1 ± 2,3 % using DCV and 98,8 ± 2,2 % using DPV at BDD (compared with the...
Keywords: acyclovir; boron-doped diamond electrode,glassy carbon electrode; oxidation; voltammetry; aciklovir; bórom dopovaná diamantová elektroda; elektroda ze skelného uhlíku; oxidace; voltametrie

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/70076

Permalink: http://www.nusl.cz/ntk/nusl-339203


The record appears in these collections:
Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses
 Record created 2017-06-19, last modified 2022-03-04


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