Original title: Structural biology approach to biotechnological problems
Authors: IERMAK, Iuliia
Document type: Doctoral theses
Year: 2017
Language: eng
Abstract: Enhancement of the catalytic performance of various enzymes in the reaction systems requires advanced studies of enzymes' structure-function relationships. The obtained information is used to engineer favourable enzyme variants exhibiting beneficial properties for applications in biotechnology. In the thesis X-ray crystallographic analysis was successfully employed for the structure-functional characterization of the glyceraldehyde dehydrogenase from thermophilic bacterium Thermoplasma acidophilum (TaAlDH). This enzyme is one of the key enzymes in the cell-free system for the production of ethanol or isobutanol from glucose. The second part of the thesis describes optimization by structure-guided engineering of the haloalkane dehalogenase LinB from the soil bacterium Sphingobium japonicum UT26, used for the effective degradation of halogenated environmental pollutants.
Citation: IERMAK, Iuliia. Structural biology approach to biotechnological problems. České Budějovice, 2017. disertační práce (Ph.D.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/35687

Permalink: http://www.nusl.cz/ntk/nusl-316032


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Doctoral theses