Original title: Vrozená imunita a cirkulující monocyty - význam a funkce v patogenezi celiakie.
Translated title: The innate immunity and circulating monocytes - their significance and function in pathogenesis of coeliac disease.
Authors: Němečková, Iva ; Daňková, Pavlína (advisor) ; Palová Jelínková, Lenka (referee)
Document type: Master’s theses
Year: 2012
Language: cze
Abstract: 8 Abstract Introduction: Celiac disease is indentified as the loss of oral tolerance to gluten, it is an organ-specific autoimmune disease in which both, adaptive and innate immunity participate. Monocytes are important part of immune system; they have many functions and express very diverse membrane receptors including Toll-like receptors (TLRs). TLRs are involved in the innate immune response, specifically TLR2 and TLR4 are crucial for recognition of bacterial components and TLR7 recognizes virus's ssRNA. Monocytes also produce prolaktin (PRL), which acts as a cytokine that modulates immune responses. To clarify the role of innate immunity and circulating monocytes in pathogenesis of celiac disease, we focused on changes in expression of selected Toll-like receptors (TLR2, TLR4, TLR7), prolactin, some pro- a anti-inflammatory cytokines (TNF-α, IL-6, IL-12, IL-10). We monitored the influence of the SNP - 1149 G/T on the expression of PRL mRNA. Another objective of this work was the introduction and optimization of in vitro methods for cultivation and stimulation of peripheral monocytes. Material and Methods: This pilot study includes 21 patients with celiac disease and 40 healthy controls. For determination of mRNA levels of the studied genes we isolated RNA from monocytes that were isolated by...
Keywords: celiac disease; cytokines; monocytes; prolactin; Toll-like receptors; celiakie; cytokiny; monocyty; prolaktin; Toll-like receptory

Institution: Charles University Faculties (theses) (web)
Document availability information: Available in the Charles University Digital Repository.
Original record: http://hdl.handle.net/20.500.11956/48238

Permalink: http://www.nusl.cz/ntk/nusl-312524


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Universities and colleges > Public universities > Charles University > Charles University Faculties (theses)
Academic theses (ETDs) > Master’s theses