Original title: Dynamic modification of proteins for fluorometric detection in CZE
Authors: Horká, Marie ; Šlais, Karel
Document type: Papers
Conference/Event: International Symposium Separations in the BioSciences /2./, Praha (CZ), 2001-09-17 / 2001-09-20
Year: 2001
Language: eng
Abstract: The separation techniques employing fluorescence detection are sensitive and selective so they have been often applied for the trace analysis of biological samples. The commonly used derivatization of proteins can lower the detection limits; however, they can change the acido-basic properties and mobilities when compared to the native species. Recently, we have used the colored tenside as a buffer additive for the photometric detection of proteins in the UV region. The selectivity, efficiency and resolution of CZE separation using this dye were found to be similar to the CZE with SDS as the additive. In this study, the ampiphilic fluorescent compound is suggested as a buffer additive in CZE for dynamic modification of the sample of several proteins. Using the deuterium lamp for the excitation in the UV region for the on column fluorometric detection, the amol minimum detectable amounts of the proteins sampled on the CZE capillary were achieved.
Keywords: capillary zone electrophoresis; dynamic modification; fluorometric detection
Project no.: CEZ:AV0Z4031919 (CEP), IAA4031901 (CEP)
Funding provider: GA AV ČR
Host item entry: 2nd International Symposium Separations in the BioSciences. SBS 2001, ISBN 80-7080-437-8

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0059618

Permalink: http://www.nusl.cz/ntk/nusl-24526


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Research > Institutes ASCR > Institute of Analytical Chemistry
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