Original title: The relationship between the modular structure of eucaryotic-like serine/threonine protein kinase StkP of Streptococcus pneumoniae and its function
Translated title: Vztah mezi modulární strukturou serin/threonin proteinkinasy eukaryontního typu StkP z Streptococcus pneumoniae a její funkcí
Authors: Pallová, Petra ; Přenosilová, Lenka ; Nováková, Linda ; Branny, Pavel
Document type: Papers
Conference/Event: European Congress of Clinical Microbiology and Infectious Diseases /14./, Praha (CZ), 2004-05-01 / 2004-05-04
Year: 2004
Language: eng
Abstract: [eng] [cze]
In order to determine a cellular localization of protein kinase StkP we prepared its epitope-labelled form expressed in S. pneumoniae. The full-length form of StkP was detected with monoclonal antibody in the membrane fraction. In vitro autophosphorylation assays confirmed this finding and phosphorylated product of corresponding mass was detected in the membranes. These results clearly showed that StkP is a membrane-associated protein. Byla určena buněčná lokalizace proteinkinasy StkP pomocí integrace do genomu S. pneumoniae jejího genu označeného epitopem. Nativní forma StkP byla detekována monoklonální protilátkou v membránové frakci. Tento výsledek byl ověřen in vitro autofosforylací a fosforylovaný produkt odpovídající velikosti byl detekován v membránách.
Keywords: ser/thr; stkp
Project no.: CEZ:AV0Z5020903 (CEP)
Host item entry: European Congress of Clinical Microbiology and Infectious Diseases /14./

Institution: Institute of Microbiology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0007911

Permalink: http://www.nusl.cz/ntk/nusl-19278


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Research > Institutes ASCR > Institute of Microbiology
Conference materials > Papers