Original title: Microarray-based identification of genes and 2-D gel analysis of proteins regulated by Ser/Thr protein kinase of Streptococcus pneumoniae
Translated title: Identifikace genů a proteinů, regulovaných Ser/Thr proteinkinasou Streptococcus pneumoniae, mikročipovou metodou a analýzou 2-D gelů
Authors: Přenosilová, Lenka ; Nováková, Linda ; Branny, Pavel
Document type: Papers
Conference/Event: European Congress of Clinical Microbiology and Infectious Diseases /14./, Praha (CZ), 2004-05-01 / 2004-05-04
Year: 2004
Language: eng
Abstract: [eng] [cze]
To identify the genes whose expression is controlled by protein kinase StkP we examined the transcription profile of DstkP mutant by DNA microarray technology. The transcript analysis revealed the StkP-dependent expression of many potential target loci. Out of 2,300 genes evaluated, 49 were found to be specifically and strongly upregulated in the DstkP strain, whereas 48 were downregulated. The data obtained from transcription profile were further extended by proteomic studies and mass spectrometry. K identifikaci genů, jejichž exprese je kontrolována funkcí proteinkinasy StkP byla použita metoda DNA čipů. Analýza transkripčních profilů prokázala, že z celkového počtu 2300 genů jich 49 bylo specificky indukováno a 48 reprimováno v mutantu stkP. Výsledky transkriptomové analýzy byly rozšířeny analýzou proteomů a hmotnostní spektrometrii.
Keywords: dna; pcr
Project no.: CEZ:AV0Z5020903 (CEP)
Host item entry: European Congress of Clinical Microbiology and Infectious Diseases /14./

Institution: Institute of Microbiology AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0007910

Permalink: http://www.nusl.cz/ntk/nusl-19277


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Research > Institutes ASCR > Institute of Microbiology
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