Original title: Effect of H- and O-termination of nanocrystalline diamond films on cell adhesion and osteogenic differentiation
Authors: Lišková, Jana ; Babchenko, Oleg ; Varga, Marián ; Kromka, Alexander ; Hadraba, Daniel ; Švindrych, Zdeněk ; Bačáková, Lucie
Document type: Papers
Conference/Event: International Conference NANOCON /6./, Brno (CZ), 2014-11-05 / 2014-11-07
Year: 2014
Language: eng
Abstract: In order to control the hydrophobic or hydrophilic character, the NCD film surfaces were grafted by hydrogen atoms (H-termination) or by oxygen atoms (O-termination). Human mesenchymal stem cells and primary human osteoblasts were used for biological studies on H- and O-terminated NCD films. We found that cells cultivated on O-terminated NCD films exhibit better adhesion than H-terminated NCD films. In addition, the expression of osteogenic cell markers collagen and alkaline phosphatase analysed by Realtime PCR and immunostaining was higher on O-terminated films.
Keywords: MSC; nanocrystalline diamond film; osteoblast; paxillin; type I collagen
Project no.: GA14-04790S (CEP), EE2.3.30.0025, ED1.1.00/02.0109, CZ.1.05/1.1.00/02.0109
Funding provider: GA ČR, GA MŠk, GA MŠk, MSMT
Host item entry: NANOCON 2014 Conference Proceedings, ISBN 978-80-87294-55-0

Institution: Institute of Physics AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0243399

Permalink: http://www.nusl.cz/ntk/nusl-180621


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Research > Institutes ASCR > Institute of Physics
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