Název: Electrochemical Biosensors Based on Enzymatic Reactor with Amalgam Powder
Autoři: Josypčuk, Bohdan ; Barek, J. ; Josypčuk, Oksana
Typ dokumentu: Příspěvky z konference
Konference/Akce: Moderní Elektrochemické Metody /34./, Jetřichovice (CZ), 2014-05-19 / 2014-05-23
Rok: 2014
Jazyk: eng
Abstrakt: Enzymatic reactor based on the powder of silver solid amalgam was suggested as the main part of biosensors in flow systems for the first time. 4-aminothiophenol, glutaraldehyde and enzyme were gradually bonded to the amalgam surface. Large surface of the fine amalgam particles maintains a big quantity of enzyme molecules. Biosensors were tested with enzymes ascorbate oxidase, glucose oxidase, catalase, tyrosinase and laccase. Electrochemical detection of the oxygen concentration change (for the first 3 enzymes) or the quinones concentration (tyrosinase and laccase) in the measured solution was done amperometrically by means of the tubular detector of silver solid amalgam. The current response of each biosensor was optimized with respect to the detection potential, the flow rate of the mobile phase, the injection volume and the enzymatic reactor volume. Under the found optimum conditions, concentration dependence and some statistical parameters of repeated measurements (relative standard deviation (RSD) for the studied enzymes was in the range 0.81 – 2.1 %) were measured. Biosensor with the ascorbate oxidase reactor was used for determination of ascorbic acid in the vitamin tablet Celaskon®. Results of the analysis were in good agreement with the contents of ascorbic acid declared by manufacturer and the RSD of these analyses was 2.0 %.
Klíčová slova: Amperometry; Electrochemical biosensors; Flow analysis
Číslo projektu: GAP206/11/1638 (CEP), GAP208/12/1645 (CEP)
Poskytovatel projektu: GA ČR, GA ČR
Zdrojový dokument: Sborník přednášek mezinárodní odborné konference XXXIV. Moderní Elektrochemické Metody, ISBN 978-80-905221-2-1

Instituce: Ústav fyzikální chemie J. Heyrovského AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v příslušném ústavu Akademie věd ČR.
Původní záznam: http://hdl.handle.net/11104/0234056

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-173884


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav fyzikální chemie J. Heyrovského
Konferenční materiály > Příspěvky z konference