Original title: High pressure modification of the simple automated liquid chromatographic system for splitless nano column gradient separations
Authors: Šesták, Jozef ; Kahle, Vladislav
Document type: Papers
Conference/Event: CECE Junior 2013, Brno (CZ), 2013-11-12 / 2013-11-13
Year: 2013
Language: eng
Abstract: Our simple liquid chromatographic system for splitless gradient nanocolumn separations at backpressure up to 50 bars was modified to run analysis under the pressure up to 300 bars. Programmable syringe pump was equipped with a 250-L high-pressure syringe and mobile phase gradients were created and stored in relatively long capillary. Available linear gradients of volumes from 5 to 50 L traced by uracil are presented. Sample introduction was performed as separate step and real large volume of sample (tens of microliters) could be injected and preconcentrated on-column. Relative standard deviation of retention times and peak areas were received (RSD < 0.3 % and RSD < 5 % respectively).
Keywords: gradient separation; liquid chromatography; nano column gradient separations
Project no.: VG20112015021 (CEP)
Funding provider: GA MV
Host item entry: Chemické Listy. Roč. 107, Issue s3., ISSN 0009-2770

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available on demand via the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0229100

Permalink: http://www.nusl.cz/ntk/nusl-170564


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