Original title: Multi-step synthesis of caspase-3 sensor based on Förster resonance energy transfer
Authors: Lišková, Marcela ; Klepárník, Karel ; Pazdera, P. ; Foret, František
Document type: Papers
Conference/Event: CECE Junior 2013, Brno (CZ), 2013-11-12 / 2013-11-13
Year: 2013
Language: eng
Abstract: Programmed cell death or apoptosis is regulated process of cell suicide. The central role in apoptosis play cysteine proteases called caspases. Caspases recognize tetra-peptide sequences Asp-Glu-Val-Asp (DEVD) on their substrates and hydrolyze peptide bonds after aspartic acid residues. Various techniques for the determination of caspase-3 are commercially available e.g. Enzyme Linked Immuno-Sorbent Assay (ELISA), Western blotting or flow cytometric analysis. The products of the cleavage can be detected by spectrophotometry, fluorimetry, chemiluminescence (CL) or ELISA. In this work, we suggested fluorescent sensor based on Förster Resonance Energy Transfer (FRET) to determine caspase-3 in cell nucleus or cytoplasm by apoptosis for very fast analysis by fluorescence microscopy without sample destroying.
Keywords: apoptotic cells; caspase-3; Förster resonance energy transfer
Project no.: EE2.3.20.0182, GAP301/11/2055 (CEP), GBP206/12/G014 (CEP), GAP206/11/2377 (CEP)
Funding provider: GA MŠk, GA ČR, GA ČR, GA ČR
Host item entry: Chemické Listy. Roč. 107, Issue s3., ISSN 0009-2770

Institution: Institute of Analytical Chemistry AS ČR (web)
Document availability information: Fulltext is available on demand via the digital repository of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0228836

Permalink: http://www.nusl.cz/ntk/nusl-170552


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Research > Institutes ASCR > Institute of Analytical Chemistry
Conference materials > Papers