Original title: Very low energy STEM for biology
Authors: Frank, Luděk ; Nebesářová, Jana ; Vancová, Marie ; Paták, Aleš ; Müllerová, Ilona
Document type: Papers
Conference/Event: Mikroskopie 2013, Lednice (CZ), 2013-05-13 / 2013-05-14
Year: 2013
Language: eng
Abstract: Examination of tissue sections with transmitted electrons has been performed at energies of hundreds and tens of eV with thicknesses of sections of 10 nm or less. This was possible by employing the cathode lens principle working without lowest energy limitations with the help of biasing the sample to a high negative potential. The reflected and transmitted electrons were attracted with the same electric field to earthed detectors situated above and below the sample. Very high image contrasts have been obtained even for samples free of any heavy metal salts for contrast enhancement.
Keywords: cathode lens; tissue sections; ultra-low-energy STEM
Project no.: TE01020118 (CEP), GAP108/11/2270 (CEP), ED0017/01/01
Funding provider: GA TA ČR, GA ČR, GA MŠk
Host item entry: Mikroskopie 2013

Institution: Institute of Scientific Instruments AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0229236

Permalink: http://www.nusl.cz/ntk/nusl-170290


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Research > Institutes ASCR > Institute of Scientific Instruments
Conference materials > Papers