Original title: Open-tubular capillary electrochromatography with bare gold nanoparticles-based stationary phase applied to separation of trypsin digested proteins
Authors: Mikšík, Ivan ; Lacinová, Kateřina ; Zmatlíková, Zdeňka ; Sedláková, Pavla ; Král, V. ; Sýkora, D. ; Řezanka, P. ; Kašička, Václav
Document type: Papers
Conference/Event: International Symposium Advances in Chromatography and Electrophoresis and Chiranal, Olomouc (CZ), 2012-06-11 / 2012-06-14
Year: 2012
Language: eng
Abstract: Open-tubular capillary electrochromatography (OT-CEC) using bare gold nanoparticles-based stationary phase has been applied to separation of tryptic peptide fragments of native and glycated proteins, bovine serum albumin and human transferrin. OT-CEC with bare gold nanoparticles stationary phase was found to be a suitable technique for separation of complex peptide mixtures originating from enzymatic (tryptic) digestion of native and glycated bovine serum albumin and human transferrin, and for glycation studies of these proteins.
Keywords: glycation of proteins; gold nanoparticles; open-tubular capillary electrochromatography
Project no.: CEZ:AV0Z50110509 (CEP), CEZ:AV0Z40550506 (CEP), GA203/09/0675 (CEP), GAP206/12/0453 (CEP)
Funding provider: GA ČR, GA ČR
Host item entry: Acta Universitatis Palackianae Olomucensis. Facultas Rerum Naturalium. Chemica 50S, ISBN 978-80-244-3115-4, ISSN 0232-0061

Institution: Institute of Organic Chemistry and Biochemistry AS ČR (web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences.
Original record: http://hdl.handle.net/11104/0216665

Permalink: http://www.nusl.cz/ntk/nusl-136060


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Research > Institutes ASCR > Institute of Organic Chemistry and Biochemistry
Conference materials > Papers